The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Trimethoprim, (5-[(3,4,5-trimethoxyphenyl)methyl]pyrimidine-2,4-diamine), is a pyrimidine inhibitor of dihydrofolate reductase and is an antibacterial related to pyrimethamine. The interference with folic acid metabolism may cause a depression of hematopoiesis. It is potentiated by sulfonamides and the trimethoprim-sulfamethoxazole combination is the form most often used. It is sometimes used alone as an antimalarial. Trimethoprim resistance has been reported. It has a molecular weight of 290.31772 g/mol and molecular formula: C14H18N4O3.