Anti-TRIOBP antibody - C-terminal (ab151320)
Key features and details
- Rabbit polyclonal to TRIOBP - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TRIOBP antibody - C-terminal
See all TRIOBP primary antibodies -
Description
Rabbit polyclonal to TRIOBP - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1833 and 2061 of Human TRIOBP (Uniprot: Q9H2D6).
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Positive control
- A549, HeLa, HepG2, HCT116 and NIH 3T3 whole cell lysates; Human MDA MB 157 xenograft tissue; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 261 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 10mM Citrate buffer
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 261 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 10mM Citrate buffer |
ICC/IF
1/100 - 1/1000. |
Target
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Relevance
TRIOBP is a protein that interacts with trio, which is involved with neural tissue development and controlling actin cytoskeleton organization, cell motility and cell growth. This trio binding protein also associates with F actin and stabilizes F actin structures. -
Cellular localization
Nucleus. Cytoplasm; cytoskeleton. Note: Localized to F-actin in a periodic pattern. -
Database links
- Entrez Gene: 511680 Cow
- Entrez Gene: 11078 Human
- Entrez Gene: 110253 Mouse
- Omim: 609761 Human
- SwissProt: Q9H2D6 Human
- SwissProt: Q99KW3 Mouse
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Alternative names
- DFNB28 antibody
- KIAA1662 antibody
- Protein TARA antibody
see all
Images
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All lanes : Anti-TRIOBP antibody - C-terminal (ab151320) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 261 kDa
10% SDS PAGE -
Anti-TRIOBP antibody - C-terminal (ab151320) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 261 kDa
10% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human MDA-MB-157 xenograft tissue labeling TRIOBP with ab151320 at 1/100 dilution.
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Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling TRIOBP using ab151320 at 1/200 dilution. Right image shows cells co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab151320 has been referenced in 1 publication.
- Jones DK et al. Localization and functional consequences of a direct interaction between TRIOBP-1 and hERG proteins in the heart. J Cell Sci 131:N/A (2018). PubMed: 29507111