Anti-TRIOBP antibody - C-terminal (ab151320)

Overview

  • Product name
    Anti-TRIOBP antibody - C-terminal
    See all TRIOBP primary antibodies
  • Description
    Rabbit polyclonal to TRIOBP - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1833 and 2061 of Human TRIOBP (Uniprot: Q9H2D6).

  • Positive control
    • A549, HeLa, HepG2, HCT116 and NIH 3T3 whole cell lysates; Human MDA MB 157 xenograft tissue; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab151320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 261 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 10mM Citrate buffer
ICC/IF 1/100 - 1/1000.

Target

  • Relevance
    TRIOBP is a protein that interacts with trio, which is involved with neural tissue development and controlling actin cytoskeleton organization, cell motility and cell growth. This trio binding protein also associates with F actin and stabilizes F actin structures.
  • Cellular localization
    Nucleus. Cytoplasm; cytoskeleton. Note: Localized to F-actin in a periodic pattern.
  • Database links
  • Alternative names
    • DFNB28 antibody
    • KIAA1662 antibody
    • Protein TARA antibody
    • TARA antibody
    • Tara like protein antibody
    • TRIO and F actin binding protein antibody
    • Trio and filamentous actin binding protein antibody
    • Trio associated repeat on actin antibody
    see all

Images

  • All lanes : Anti-TRIOBP antibody - C-terminal (ab151320) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 261 kDa



    10% SDS PAGE
  • Anti-TRIOBP antibody - C-terminal (ab151320) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg

    Predicted band size: 261 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human MDA-MB-157 xenograft tissue labeling TRIOBP with ab151320 at 1/100 dilution.
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling TRIOBP using ab151320 at 1/200 dilution. Right image shows cells co-stained with Hoechst 33342.

References

ab151320 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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