Anti-Triosephosphate isomerase antibody (ab58327)


  • Product nameAnti-Triosephosphate isomerase antibody
    See all Triosephosphate isomerase primary antibodies
  • Description
    Mouse monoclonal to Triosephosphate isomerase
  • Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-250 of Human Triosephosphate isomerase



Our Abpromise guarantee covers the use of ab58327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 27 kDa.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • RelevanceTriosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.
  • Cellular localizationCytoplasmic and Nuclear; extracellular vesicle exosome; extracellular space.
  • Database links
  • Alternative names
    • epididymis secretory protein Li 49 antibody
    • HEL-S-49 antibody
    • MGC88108 antibody
    • TIM antibody
    • TPI 1 antibody
    • TPI antibody
    • TPI1 antibody
    • TPID antibody
    • Triose phosphate isomerase 1 antibody
    • Triose phosphate isomerase antibody
    • Triosephosphate isomerase 1 antibody
    • Triosephosphate isomerase antibody
    see all

Anti-Triosephosphate isomerase antibody images

  • Overlay histogram showing Jurkat cells stained with ab58327 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58327, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Predicted band size : 27 kDa
    Triosephosphate isomerase antibody (ab58327) at 1ug/lane + HepG2 cell lysate at 25ug/lane.
  • ICC/IF image of ab58327 stained Mcf7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58327, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Triosephosphate isomerase antibody (ab58327)

ab58327 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Cell lysate - other (Primary endothelial cells)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 150 µg
Treatment 0.5% BSA for 24 h
Specification Primary endothelial cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Jul 27 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT116)
Loading amount 10 µg
Specification HCT116
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Oct 13 2010