• Product nameAnti-Triosephosphate isomerase antibody
    See all Triosephosphate isomerase primary antibodies
  • Description
    Rabbit polyclonal to Triosephosphate isomerase
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 187-249 of Human Triosephosphate isomerase (NP_000356).

  • Positive control
    • WB: 293T, HeLaS3, HepG2 and Raji cell lysates; A431, H1299, whole cell lysates. IHC-P: SNU16 tissue



Our Abpromise guarantee covers the use of ab96696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 27 kDa.
IHC-P 1/100.


  • RelevanceTriosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.
  • Cellular localizationCytoplasmic and Nuclear; extracellular vesicle exosome; extracellular space.
  • Database links
  • Alternative names
    • epididymis secretory protein Li 49 antibody
    • HEL-S-49 antibody
    • MGC88108 antibody
    • TIM antibody
    • TPI 1 antibody
    • TPI antibody
    • TPI1 antibody
    • TPID antibody
    • Triose phosphate isomerase 1 antibody
    • Triose phosphate isomerase antibody
    • Triosephosphate isomerase 1 antibody
    • Triosephosphate isomerase antibody
    see all

Anti-Triosephosphate isomerase antibody images

  • All lanes : Anti-Triosephosphate isomerase antibody (ab96696) at 1/2000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 27 kDa
  • ab96696, at a 1/100 dilution, staining Triosephosphate isomerase in paraffin embedded SNU16 by Immunohistochemical analysis.

References for Anti-Triosephosphate isomerase antibody (ab96696)

This product has been referenced in:
  • Peng X  et al. Autophagy promotes paclitaxel resistance of cervical cancer cells: involvement of Warburg effect activated hypoxia-induced factor 1-a-mediated signaling. Cell Death Dis 5:e1367 (2014). WB ; Human . Read more (PubMed: 25118927) »
  • Mukai C  et al. Biomimicry enhances sequential reactions of tethered glycolytic enzymes, TPI and GAPDHS. PLoS One 8:e61434 (2013). WB ; Mouse . Read more (PubMed: 23626684) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Skeletal Muscle)
Loading amount 20 µg
Specification Skeletal Muscle
Gel Running Conditions Non-reduced Denaturing (10 %)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mrs. Maria Insenser

Verified customer

Submitted Jan 27 2012