Anti-Triosephosphate isomerase antibody (ab96696)
Key features and details
- Rabbit polyclonal to Triosephosphate isomerase
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Triosephosphate isomerase antibody
See all Triosephosphate isomerase primary antibodies -
Description
Rabbit polyclonal to Triosephosphate isomerase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 187-249 of Human Triosephosphate isomerase (NP_000356).
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Positive control
- WB: 293T, HeLaS3, HepG2 and Raji cell lysates; A431, H1299, whole cell lysates. IHC-P: SNU16 tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 27 kDa.
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IHC-P |
1/100.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 27 kDa. |
IHC-P
1/100. |
Target
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Relevance
Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. -
Cellular localization
Cytoplasmic and Nuclear; extracellular vesicle exosome; extracellular space. -
Database links
- Entrez Gene: 7167 Human
- Omim: 190450 Human
- SwissProt: P60174 Human
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Alternative names
- epididymis secretory protein Li 49 antibody
- HEL-S-49 antibody
- MGC88108 antibody
see all
Images
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All lanes : Anti-Triosephosphate isomerase antibody (ab96696) at 1/2000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 27 kDa
12% SDS PAGE -
ab96696, at a 1/100 dilution, staining Triosephosphate isomerase in paraffin embedded SNU16 (human) by Immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab96696 has been referenced in 8 publications.
- Coulibaly A et al. STAT3 governs the HIF-1a response in IL-15 primed human NK cells. Sci Rep 11:7023 (2021). PubMed: 33782423
- Wu L et al. Niche-Selective Inhibition of Pathogenic Th17 Cells by Targeting Metabolic Redundancy. Cell 182:641-654.e20 (2020). PubMed: 32615085
- Velásquez SY et al. Innate Cytokine Induced Early Release of IFN? and CC Chemokines from Hypoxic Human NK Cells Is Independent of Glucose. Cells 9:N/A (2020). PubMed: 32192004
- Gao W et al. Targeting oxidative pentose phosphate pathway prevents recurrence in mutant Kras colorectal carcinomas. PLoS Biol 17:e3000425 (2019). PubMed: 31461438
- Lone SN et al. Triose-phosphate isomerase is a novel target of miR-22 and miR-28, with implications in tumorigenesis. J Cell Physiol 233:8919-8929 (2018). PubMed: 29856481
- Rai V et al. Real-time iTRAQ-based proteome profiling revealed the central metabolism involved in nitrogen starvation induced lipid accumulation in microalgae. Sci Rep 7:45732 (2017). PubMed: 28378827
- Peng X et al. Autophagy promotes paclitaxel resistance of cervical cancer cells: involvement of Warburg effect activated hypoxia-induced factor 1-a-mediated signaling. Cell Death Dis 5:e1367 (2014). WB ; Human . PubMed: 25118927
- Mukai C et al. Biomimicry enhances sequential reactions of tethered glycolytic enzymes, TPI and GAPDHS. PLoS One 8:e61434 (2013). WB ; Mouse . PubMed: 23626684