Overview

  • Product name
  • Description
    Goat polyclonal to TRIP
  • Tested applications
    Suitable for: ICC, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    SLFQAKLDTFLWS

    , corresponding to C terminal amino acids 457-469 of Human TRIP.

  • General notes
    GenBank Accession Number – NP_005870.

Properties

Applications

Our Abpromise guarantee covers the use of ab4533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 4 - 6 µg/ml.
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 53 kDa. additonal band of unkown identity was also concistently observed at 38kDa. This band was successfully blocked by incubation with the immunizing peptide.

Target

  • Function
    Inhibits activation of NF-kappa-B mediated by TNF.
  • Sequence similarities
    Contains 1 RING-type zinc finger.
  • Cellular localization
    Cytoplasm. Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • RING finger protein 206 antibody
    • RNF206 antibody
    • TRAF Interacting Protein antibody
    • TRAF-interacting protein antibody
    • Traip antibody
    • TRAIP_HUMAN antibody
    • TRIP antibody
    see all

Images

  • Anti-TRIP antibody (ab4533) at 2 µg/ml + Human Testis lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
  • In paraffin embedded Human Spleen shows perinucxlear and textured cystoplasmic staining in selective areas.ab4533 (4µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

References

ab4533 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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