The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 4 - 6 µg/ml.
Use a concentration of 2 µg/ml. Predicted molecular weight: 53 kDa. additonal band of unkown identity was also concistently observed at 38kDa. This band was successfully blocked by incubation with the immunizing peptide.
FunctionInhibits activation of NF-kappa-B mediated by TNF.
Anti-TRIP antibody (ab4533) at 2 µg/ml + Human Testis lysate in RIPA buffer at 35 µg developed using the ECL technique
Predicted band size : 53 kDa Observed band size : 53 kDa Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry - Anti-TRIP antibody (ab4533)
In paraffin embedded Human Spleen shows perinucxlear and textured cystoplasmic staining in selective areas.ab4533 (4µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
References for Anti-TRIP antibody (ab4533)
has not yet been referenced specifically in any publications.
Publishing research using ab4533? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"