• Product name
    Anti-TRIP12 antibody
  • Description
    Rabbit polyclonal to TRIP12
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Camel, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 75 and 125 of human TRIP12 (NP_004229.1)

  • Positive control
    • HeLa whole cell lysate


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab86220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 220 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      TRIP12 (thyroid hormone receptor interactor 12) is an ATP-dependent multisubunit protein that activates the proteolytic activities of the multifunctional proteinase (20S proteasome) of the 26S complex. TRP12 specifically interacts with the ligand binding domain of the thyroid hormone receptor (in a thyroid hormone T3-independent manner) and with retinoid X receptor (RXR). TRP12 was originally identified as a factor that interacts with the thyroid hormone receptor. It was later found to also interact with AAP-BP1, a component of the NEDD8 –activating enzyme, and function as an E3 ubiquitin ligase for AAP-BP1.
    • Database links
    • Alternative names
      • KIAA0045 antibody
      • MGC138849 antibody
      • MGC138850 antibody
      • Thyroid hormone receptor interactor 12 antibody
      • Thyroid receptor interacting protein 12 antibody
      • TRIP 12 antibody
      see all

    Anti-TRIP12 antibody images

    • All lanes : Anti-TRIP12 antibody (ab86220) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 5 µg
      Lane 2 : HeLa whole cell lysate at 1.5 µg
      Lane 3 : HeLa whole cell lysate at 0.5 µg

      Developed using the ECL technique

      Predicted band size : 220 kDa

      Exposure time : 3 minutes
    • Detection of human TRIP12 in Immunoprecipitates of HeLa whole cell lysate (1 mg lysate for IP, 20% of IP loaded) using ab86220 at 3 µg/mg lysate for IP, and at 0.1 µg/ml for subsequent Western blot.

      Detection: Chemiluminescence with an exposure time of 10 seconds.

    References for Anti-TRIP12 antibody (ab86220)

    This product has been referenced in:
    • Mounir Z  et al. ERG signaling in prostate cancer is driven through PRMT5-dependent methylation of the Androgen Receptor. Elife 5:N/A (2016). WB . Read more (PubMed: 27183006) »

    See 1 Publication for this product

    Product Wall

    We do not have data for mouse reactivity for this TRIP12 antibody, ab86220. The amino acid sequence of the immunogen that generated the antibody is well-conserved in the mouse protein so cross-reaction is possible, though not experimentally confirmed.<...

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    Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guaran...

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