Overview

  • Product nameAnti-TRIP12 antibody
  • Description
    Rabbit polyclonal to TRIP12
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Camel, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 75 and 125 of human TRIP12 (NP_004229.1)

  • Positive control
    • HeLa whole cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab86220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 220 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceTRIP12 (thyroid hormone receptor interactor 12) is an ATP-dependent multisubunit protein that activates the proteolytic activities of the multifunctional proteinase (20S proteasome) of the 26S complex. TRP12 specifically interacts with the ligand binding domain of the thyroid hormone receptor (in a thyroid hormone T3-independent manner) and with retinoid X receptor (RXR). TRP12 was originally identified as a factor that interacts with the thyroid hormone receptor. It was later found to also interact with AAP-BP1, a component of the NEDD8 –activating enzyme, and function as an E3 ubiquitin ligase for AAP-BP1.
    • Database links
    • Alternative names
      • KIAA0045 antibody
      • MGC138849 antibody
      • MGC138850 antibody
      • Thyroid hormone receptor interactor 12 antibody
      • Thyroid receptor interacting protein 12 antibody
      • TRIP 12 antibody
      see all

    Anti-TRIP12 antibody images

    • All lanes : Anti-TRIP12 antibody (ab86220) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 5 µg
      Lane 2 : HeLa whole cell lysate at 1.5 µg
      Lane 3 : HeLa whole cell lysate at 0.5 µg

      Developed using the ECL technique

      Predicted band size : 220 kDa


      Exposure time : 3 minutes
    • Detection of human TRIP12 in Immunoprecipitates of HeLa whole cell lysate (1 mg lysate for IP, 20% of IP loaded) using ab86220 at 3 µg/mg lysate for IP, and at 0.1 µg/ml for subsequent Western blot.

      Detection: Chemiluminescence with an exposure time of 10 seconds.

    References for Anti-TRIP12 antibody (ab86220)

    ab86220 has not yet been referenced specifically in any publications.

    Product Wall

    We do not have data for mouse reactivity for this TRIP12 antibody, ab86220. The amino acid sequence of the immunogen that generated the antibody is well-conserved in the mouse protein so cross-reaction is possible, though not experimentally confirmed.<...

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    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guaran...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"