Anti-TRIP13 antibody - Carboxyterminal end (ab64964)

Overview

  • Product name
    Anti-TRIP13 antibody - Carboxyterminal end
    See all TRIP13 primary antibodies
  • Description
    Rabbit polyclonal to TRIP13 - Carboxyterminal end
  • Specificity
    Detects endogenous levels of total TRIP13 protein.
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from C terminal of human TRIP13.

  • Positive control
    • Extracts from LOVO cells and NIH 3T3 cells IHC-P: Human lunf FFPE tissue sections.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).

Target

  • Sequence similarities
    Belongs to the AAA ATPase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 16E1-BP antibody
    • 16E1BP antibody
    • Homo sapiens HPV16 E1 protein binding protein mRNA complete cds antibody
    • HPV16 E1 protein binding protein antibody
    • HPV16 E1 protein-binding protein antibody
    • Human papillomavirus type 16 E1 protein binding protein antibody
    • Human papillomavirus type 16 E1 protein-binding protein antibody
    • Pachytene checkpoint protein 2 homolog antibody
    • PCH2 antibody
    • Thyroid hormone receptor interactor 13 antibody
    • Thyroid receptor interacting protein 13 antibody
    • Thyroid receptor-interacting protein 13 antibody
    • TR-interacting protein 13 antibody
    • TRIP-13 antibody
    • Trip13 antibody
    • TRP13_HUMAN antibody
    see all

Images

  • IHC image of TRIP13 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64964, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-TRIP13 antibody - Carboxyterminal end (ab64964) at 1/500 dilution

    Lane 1 : extracts from LOVO cells without immunizing peptide
    Lane 2 : extract from NIH 3T3 cells, without immunizing peptide
    Lane 3 : extract from NIH 3T3 cells with immunizing peptide


    Predicted band size : 49 kDa
    Observed band size : 49 kDa
    The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.

References

This product has been referenced in:
  • Pressly JD  et al. TRIP13-deficient tubular epithelial cells are susceptible to apoptosis following acute kidney injury. Sci Rep 7:43196 (2017). Read more (PubMed: 28256593) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (12% SDS-PAGE)
Sample
Human Cell lysate - whole cell (NIH 3T3)
Specification
NIH 3T3
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

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Verified customer

Submitted Aug 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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