Anti-CSN2 antibody (ab10426)
Key features and details
- Rabbit polyclonal to CSN2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-CSN2 antibody
See all CSN2 primary antibodies -
Description
Rabbit polyclonal to CSN2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
Immunogen
Synthetic peptide corresponding to CSN2. The epitope recognized by ab10426 maps to a region between residues 1 and 50 of human CSN2.
Database link: P61201 -
Positive control
- WB: HEK293T cells.
-
General notes
CSN2 is a subunit of the JUN activation-domain binding protein 1 (JAB1), containing signalosome complex (or COP9 complex) which has kinase activity and phosphorylates C-JUN. The signalosome complex may play a role in signal transduction.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. -
Primary antibody notes
CSN2 is a subunit of the JUN activation-domain binding protein 1 (JAB1), containing signalosome complex (or COP9 complex) which has kinase activity and phosphorylates C-JUN. The signalosome complex may play a role in signal transduction. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
|
|
IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa). |
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Involved in early stage of neuronal differentiation via its interaction with NIF3L1. -
Sequence similarities
Belongs to the CSN2 family.
Contains 1 PCI domain. -
Post-translational
modificationsPhosphorylated by CK2 and PKD kinases. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 430917 Chicken
- Entrez Gene: 34225 Drosophila melanogaster
- Entrez Gene: 9318 Human
- Entrez Gene: 12848 Mouse
- Entrez Gene: 100125956 Pig
- Entrez Gene: 261736 Rat
- Entrez Gene: 548537 Xenopus tropicalis
- Entrez Gene: 415145 Zebrafish
see all -
Alternative names
- ALIEN antibody
- Alien Drosophila himolog of antibody
- Alien homolog antibody
see all
Images
-
Anti-CSN2 antibody (ab10426) at 1/10000 dilution + HEK293T Whole cell lysate at 50 µg
Predicted band size: 52 kDaDetection: Chemiluminescence with an exposure time of 3 minutes.
-
IHC-P section of human prostate carcinoma (left) and mouse squamous cell carcinoma (right). Epitope retrieval with citrate buffer pH 6.0
Antibody used at a dilution of 1:5,000 (0.2μg/ml) and 1:1,000 (1μg/ml).
Detection: DAB
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab10426 has been referenced in 1 publication.
- Oh W et al. Jab1 induces the cytoplasmic localization and degradation of p53 in coordination with Hdm2. J Biol Chem 281:17457-65 (2006). PubMed: 16624822