Overview

  • Product nameAnti-TRIP230 antibody
  • Description
    Rabbit polyclonal to TRIP230
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    A region between residues 25 and 75 of TRIP230

  • Positive control
    • Whole cell lysates from HeLa and 293T cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab72223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 238 kDa (predicted molecular weight: 228 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • RelevanceTRIP230 was identified through its ability to interact functionally with thyroid hormone receptor-beta. It has also been found in association with the Golgi apparatus and microtubules. TRIP230 binds the ligand binding domain of the thyroid receptor (THRB) in the presence of triiodothyronine and enhances THRB-modulated transcription. This protein is probably involved in maintaining cis-Golgi structure.
  • Cellular localizationCell Membrane and Golgi Apparatus
  • Database links
  • Alternative names
    • CEV 14 antibody
    • CEV14 antibody
    • Clonal evolution related gene on chromosome 14 antibody
    • GMAP 210 antibody
    • GMAP210 antibody
    • Golgi associated microtubule binding protein 210 antibody
    • Thyroid hormone receptor interactor 11 antibody
    • Thyroid receptor interacting protein 11 antibody
    • TRIP 11 antibody
    • TRIP 230 antibody
    • TRIP11 antibody
    see all

Anti-TRIP230 antibody images

  • All lanes : Anti-TRIP230 antibody (ab72223) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 228 kDa
    Observed band size : 238 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 seconds
  • Detection of Human TRIP230 by Immunoprecipitation. 1mg HeLa whole cell lysate loaded in lanes 1-3 with ab72223 at 3µg in lane 1, an antibody recognising a downstream epitope in lane 2 and control IgG in lane 3. Image shows immunoprecipitated TRIP230 detected with post IP WB, loading 20% of IP and using ab72223 at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

References for Anti-TRIP230 antibody (ab72223)

ab72223 has not yet been referenced specifically in any publications.

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