Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab70747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionRelays signals from the cell surface to the nucleus to weaken adherens junction and promote actin cytoskeleton reorganization and cell invasiveness. Involved in lysophosphatidic acid-induced cell adhesion and migration. Acts as a transcriptional coactivator for NF-kappa-B and JUN, and mediates the transrepression of these transcription factors induced by glucocorticoid receptor.
  • Tissue specificityAbundantly expressed in kidney, liver and lung. Lower levels in heart, placenta and pancreas. Expressed in colonic epithelial cells. Up-regulated in colonic tumors.
  • Sequence similaritiesBelongs to the zyxin/ajuba family.
    Contains 3 LIM zinc-binding domains.
  • DomainThe LIM zinc-binding domains mediate interaction with LPAR2 and with S.typhimurium protein sseI.
  • Post-translational
    modifications
    Phosphorylation at Tyr-55 by SRC is required for enhancement of lysophosphatidic acid-induced cell migration. Tyr-55 is dephosphorylated by PTPN13.
  • Cellular localizationCytoplasm > cytoskeleton. Cell junction > focal adhesion. Nucleus. Cytoplasm. Shuttles between nucleus and cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC10556 antibody
    • MGC10558 antibody
    • MGC29959 antibody
    • MGC3837 antibody
    • MGC4423 antibody
    • OIP-1 antibody
    • OIP1 antibody
    • OPA interacting protein 1 antibody
    • OPA-interacting protein 1 antibody
    • Thyroid hormone receptor interactor 6 antibody
    • Thyroid receptor interacting protein 6 antibody
    • Thyroid receptor-interacting protein 6 antibody
    • TR-interacting protein 6 antibody
    • TRIP-6 antibody
    • Trip6 antibody
    • TRIP6_HUMAN antibody
    • TRIP6i2 antibody
    • ZRP 1 antibody
    • ZRP-1 antibody
    • Zyxin-related protein 1 antibody
    see all

Anti-TRIP6 antibody images

  • All lanes : Anti-TRIP6 antibody (ab70747) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 50 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • 1mg whole cell lysate from HeLa cells was immunprecipitated using ab70747 at 3µg/mg of lysate. For the subsequent western blot, 20% of the immunoprecipitate was probed with ab70747 at 1ug/ml. Detection: chemiluminescence with exposure time of 10 seconds.

References for Anti-TRIP6 antibody (ab70747)

ab70747 has not yet been referenced specifically in any publications.

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