Recombinant
RabMAb

Anti-Tripeptidyl peptidase II antibody [EPR13202] (ab180177)

Overview

  • Product name
    Anti-Tripeptidyl peptidase II antibody [EPR13202]
    See all Tripeptidyl peptidase II primary antibodies
  • Description
    Rabbit monoclonal [EPR13202] to Tripeptidyl peptidase II
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Tripeptidyl peptidase II. The exact sequence is proprietary.
    Database link: P29144

  • Positive control
    • Jurkat, HeLa, HepG2, and fetal liver lysates; Human tonsil tissue; HeLa cells; Jurkat cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab180177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 138 kDa (predicted molecular weight: 138 kDa).
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance
      Tripeptidyl peptidase II is a component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway. It may be able to complement the 26S proteasome function to some extent under conditions in which the latter is inhibited. Tripeptidyl peptidase II is a mammalian peptidase that, at neutral pH, removes tripeptides from the N terminus of longer peptides. The protein has a specialized function that is essential for some MHC class I antigen presentation. The protein is a high molecular mass serine exopeptidase; the amino acid sequence surrounding the serine residue at the active site is similar to the peptidases of the subtilisin class rather than the trypsin class.
    • Cellular localization
      Cytoplasmic
    • Database links
    • Alternative names
      • TPP II antibody
      • TPP2 antibody
      • Tripeptidyl aminopeptidase antibody
      • Tripeptidyl peptidase 2 antibody
      • Tripeptidyl peptidase II antibody
      see all

    Images

    • All lanes : Anti-Tripeptidyl peptidase II antibody [EPR13202] (ab180177) at 1/1000 dilution

      Lane 1 : Jurkat lysate
      Lane 2 : HeLa lysate
      Lane 3 : HepG2 lysate
      Lane 4 : fetal liver lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 138 kDa
      Additional bands at : 138 kDa. We are unsure as to the identity of these extra bands.
    • Flow Cytometrical analysis of permeabilized Jurkat cells labeling Tripeptidyl peptidase II with ab180177 at 1/10 (red) or a rabbit IgG (negative) (green).

    • Immunofluorescent analysis of HeLa cells labeling Tripeptidyl peptidase II with ab180177 at 1/100 (red) and DAPI staining (blue).

    • Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Tripeptidyl peptidase II with ab180177 at 1/50.

    References

    ab180177 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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