TripleStain IHC kit: M&M&R on rodent tissue (DAB, HRP/Green & AP/Red) (ab183292)
Overview
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Product name
TripleStain IHC kit: M&M&R on rodent tissue (DAB, HRP/Green & AP/Red) -
Assay type
Polymer Multiplex -
Product overview
The TripleStain IHC kit (ab183292) is designed to use with two mouse antibodies and one rabbit antibody (user supplied) to detect three distinct antigens on a single mouse/rat tissue or cell sample. This kit has been tested in paraffin-embedded formalin-fixed tissues. However, it can be used on frozen specimens and freshly prepared monolayer cell smears.
Triple staining is a common method used in immunohistochemistry for the detection of three distinct antigens in a single tissue. Abcam’s TripleStain IHC Kit supplies polymer enzyme conjugates: polymer-HRP anti-mouse IgG, polymer-AP anti-mouse IgG, and polymer-HRP anti-rabbit IgG with three substrates/chromogens; DAB (brown), Emerald (green), and Permanent Red (Red). DAB (Brown) and Permanent Red (red). The TripleStain Kit is a non-biotin system avoiding non-specific background due to endogenous biotin binding. A Primer step is used to increase specificity of antibody staining. This kit has been optimized to have no cross detection when detecting two primary antibodies from the same host species using a unique blocking system.
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Tested applications
Suitable for: IHC-Fr, IHC-Pmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 72 ml 60 ml 6 ml Antibody Blocker (40x) 1 x 50ml 1 x 100ml 1 x 30ml Blocker A 2 x 18ml 1 x 60ml 1 x 6ml Blocker B 2 x 18ml 1 x 60ml 1 x 6ml DAB Chromogen (20x) 1 x 2ml 1 x 6ml 1 x 1.5ml DAB Substrate 2 x 18ml 1 x 120ml 1 x 12ml Emerald Chromogen 2 x 18ml 1 x 60ml 1 x 6ml Mouse AP Polymer 1 x 18ml 1 x 60ml 1 x 6ml Mouse HRP Polymer 2 x 18ml 1 x 60ml 1 x 6ml Mouse Primer 2 x 18ml 1 x 120ml 1 x 6ml Non-aqueous Mounting Medium 2 x 18ml 0 x 0ml 1 x 6ml Permanent Red Activator (5x) 1 x 7.2ml 1 x 24ml 1 x 3ml Permanent Red Chromogen (100x) 1 x 360µl 1 x 1.2ml 1 x 150µl Permanent Red Substrate 2 x 18ml 1 x 120ml 1 x 15ml Rabbit HRP Polymer 1 x 18ml 1 x 60ml 1 x 6ml -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab183292 has not yet been referenced specifically in any publications.