TripleStain IHC kit: M&M&R on rodent tissue (DAB, HRP/Green & AP/Red) (ab183292)

Overview

  • Product name
    TripleStain IHC kit: M&M&R on rodent tissue (DAB, HRP/Green & AP/Red)
  • Assay type
    Polymer Multiplex
  • Product overview

    The TripleStain IHC kit (ab183292) is designed to use with two mouse antibodies and one rabbit antibody (user supplied) to detect three distinct antigens on a single mouse/rat tissue or cell sample. This kit has been tested in paraffin-embedded formalin-fixed tissues. However, it can be used on frozen specimens and freshly prepared monolayer cell smears.

     

    Triple staining is a common method used in immunohistochemistry for the detection of three distinct antigens in a single tissue. Abcam’s TripleStain IHC Kit supplies polymer enzyme conjugates: polymer-HRP anti-mouse IgG, polymer-AP anti-mouse IgG, and polymer-HRP anti-rabbit IgG with three substrates/chromogens; DAB (brown), Emerald (green), and Permanent Red (Red). DAB (Brown) and Permanent Red (red). The TripleStain Kit is a non-biotin system avoiding non-specific background due to endogenous biotin binding. A Primer step is used to increase specificity of antibody staining. This kit has been optimized to have no cross detection when detecting two primary antibodies from the same host species using a unique blocking system.

  • Tested applications
    Suitable for: IHC-Fr, IHC-Pmore details

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 24 ml 72 ml 240 ml
    Antibody Blocker (40x) 2 x 15ml 1 x 50ml 1 x 100ml
    Blocker A 1 x 12ml 2 x 18ml 1 x 120ml
    Blocker B 1 x 12ml 2 x 18ml 1 x 120ml
    DAB Chromogen (20x) 1 x 1.5ml 1 x 2ml 1 x 6ml
    DAB Substrate 1 x 15ml 2 x 18ml 1 x 120ml
    Emerald Chromogen 1 x 15ml 2 x 18ml 1 x 120ml
    Mouse AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Mouse HRP Polymer 1 x 12ml 2 x 18ml 1 x 120ml
    Mouse Primer 1 x 12ml 2 x 18ml 1 x 120ml
    Non-aqueous Mounting Medium 1 x 12ml 2 x 18ml
    Permanent Red Activator (5x) 1 x 3ml 1 x 7.2ml 2 x 12ml
    Permanent Red Chromogen (100x) 1 x 150µl 1 x 360µl 1 x 1.2ml
    Permanent Red Substrate 1 x 15ml 2 x 18ml 1 x 120ml
    Rabbit HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Protocols

References

ab183292 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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