Tris Buffered Saline (TBS) Casein ELISA Reagent (ab171533)

Overview

  • Product name
    Tris Buffered Saline (TBS) Casein ELISA Reagent
    See all ELISA buffer reagents
  • Tested applications
    Suitable for: ELISAmore details
    Unsuitable for: IHC-Fr or IHC-P
  • General notes

    Abcam’s Tris Buffered Saline (TBS) Casein ELISA Reagent consists of many phosphoserine groups that function to bind Ca2+. The solution is ready for dilution to specific application requirements. ab171533 contributes to the minimization of non-specific interactions of the secondary reagents with each other and with the primary solid phase binding sites. The high blocking efficiency of casein is due to its content of small molecular weight proteins. Tris Buffered Saline (TBS), pH 7.6, is a poor buffer below pH 7.5. Tris has the disadvantages of its reactivity as a primary amine and its appreciable solubility in organic solvents, which can lead to an accumulation in the biological phase of a reaction system. To avoid complex formation with ionic species such as calcium and magnesium in blood, tris buffers are preferable over phosphate buffers.

    Storage Instructions:
    Store at room temperature. This product should be clear and colorless in appearance. To minimize the risk of contamination, a fresh working solution should be prepared each day or store unused working solutions at the recommended storage temperature. If proper technique is not performed the possibility of introducing contaminants is always possible.

    Product Use:
    ab171533 should be diluted with reagent quality water. To produce a 1X working solution, dilute 1 mL of Tris Buffered Saline (TBS) Casein ELISA Reagent in 9 mL reagent quality water. Allow working solution to equilibrate to room temperature (25°C) prior to use. To use as a blocking reagent, treat target surface by covering completely with solution. To use working solution as a diluent, dilute antigen/antibody to desired concentration and treat surface by covering completely with solution. When solution is used for coating plates are obtained at +4°C.

Properties

  • Form
    Liquid
  • Storage instructions
    Please see notes section.
  • Storage buffer
    Constituents: Casein, 40% Proprietary Component
  • Concentration information loading...

Applications

Our Abpromise guarantee covers the use of ab171533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-Fr or IHC-P.
  • References

    ab171533 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    The optimal pH range for diluted TBSC is 7.4 - 7.8 at 22oC to 28oC.

    When using 1X solution for coating plates, best results are obtained following an overnight incubation at 2oC to 8oC.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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