Anti-Tristetraprolin antibody [8B5] (ab124024)

Overview

  • Product nameAnti-Tristetraprolin antibody [8B5]
    See all Tristetraprolin primary antibodies
  • Description
    Mouse monoclonal [8B5] to Tristetraprolin
  • Tested applicationsSuitable for: IHC-P, Flow Cyt, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human Tristetraprolin produced in HEK293T cells (NP_003398).

  • Positive control
    • HEK293T cells transfected with pCMV6-ENTRY Tristetraprolin cDNA; Human colon, colon adenocarcinoma, ovary, endometrium and prostate tissues; COS7 cells transiently transfected by pCMV6-ENTRY Tristetraprolin.
  • General notesDilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

Properties

Applications

Our Abpromise guarantee covers the use of ab124024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.
WB 1/1 - 1/1000.

Target

  • FunctionmRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. Acts by specifically binding ARE-containing mRNAs and promoting their degradation. Recruits deadenylase CNOT7 (and probably the CCR4-NOT complex) via association with CNOT1. Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF). Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF).
  • Sequence similaritiesContains 2 C3H1-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylation by MAPKAPK2 increases its stability and binding to 14-3-3 proteins, leading to reduce its ARE affinity leading to inhibition of degradation of ARE-containing transcripts. Phosphorylated upon mitogen stimulation.
  • Cellular localizationNucleus. Cytoplasm. Localizes to stress granules upon energy starvation. phosphorylation by MAPKAPK2 promotes exclusion from stress granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • G0/G1 switch regulatory protein 24 antibody
    • G0S24 antibody
    • GOS24 antibody
    • Growth factor-inducible nuclear protein NUP475 antibody
    • NUP475 antibody
    • Protein TIS11A antibody
    • RNF162A antibody
    • TIS 11 antibody
    • TIS11 antibody
    • TIS11A antibody
    • Tristetraprolin antibody
    • Tristetraproline antibody
    • TTP antibody
    • TTP_HUMAN antibody
    • Zfp-36 antibody
    • ZFP36 antibody
    • Zinc finger protein 36 antibody
    • Zinc finger protein 36 homolog antibody
    • Zinc finger protein 36, C3H type, homolog (mouse) antibody
    • Zinc finger protein, C3H type, 36 homolog antibody
    see all

Anti-Tristetraprolin antibody [8B5] images

  • All lanes : Anti-Tristetraprolin antibody [8B5] (ab124024)

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY Tristetraprolin cDNA

    Lysates/proteins at 5 µg per lane.
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202049)
  • ab124024, at 1/50, staining Tristetraprolin in paraffin embedded Human colon tissue by Immunohistochemistry.
  • ab124024, at 1/50, staining Tristetraprolin in paraffin embedded Human colon adenocarcinoma tissue by Immunohistochemistry.
  • ab124024, at 1/50, staining Tristetraprolin in paraffin embedded Human ovary tissue by Immunohistochemistry.
  • ab124024, at 1/50, staining Tristetraprolin in paraffin embedded Human endometrium tissue by Immunohistochemistry.
  • ab124024, at 1/50, staining Tristetraprolin in paraffin embedded Human prostate tissue by Immunohistochemistry.
  • ab124024, at 1/100, staining Tristetraprolin in COS7 cells transiently transfected with pCMV6-ENTRY Tristetraprolin by Immunofluorescence.
  • ab124024 at 1/100 dilution staining Tristetraprolin in HEK293T cells transfected with either pCMV6-ENTRY Tristetraprolin (Red) or empty vector control plasmid (Blue) by flow cytometry.

References for Anti-Tristetraprolin antibody [8B5] (ab124024)

ab124024 has not yet been referenced specifically in any publications.

Product Wall

Thank you for providing that information and for your patience and understanding in this matter.

The information that Cedarlane provided is exactly what I needed. I have gone ahead and proceed the free of charge replacement and you will be re...

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Unfortunately the information you provided is not the PO# that Cedarlane supplied to Abcam. The PO that you supply to them is not the same as the one they supply to us. Could you please call/email Cedarlane and ask f...

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Unfortunately that PO# does not match the one that we have in our system. Is this the PO# that Cedalane told you they provided to Abcam, or is this the PO# that you provided to Cedarlane?

Before I can proce...

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Thank you for your reply.

It will not be an issue replacing the antibody even though it was purchased through Cedarlane. What I do need though is the PO# that Cedarlane supplied to Abcam and the date they placed the order. If you could contac...

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Thank you for your reply and for providing that extra information.

As the antibody is not giving you satisfactory results in human samples, I will be more than happy to replace or refund the antibody for you. We do not have any antibodies aga...

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Thank you for contacting Abcam.

The antibody ab124024 is covered under our Abpromise for six months and is guaranteed to work in western blot on human samples. We have not tested the antibody on mouse samples and you mention in your original ...

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We have 1 mouse monoclonal antibody available for which ICC/IF is a tested application. If this antibodies does detect ZFP36 in negative cells we will offer you refund.

http://www.abcam.com/Tristetraproli...

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Thank you for taking time to provide this information and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vita...

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Thank you for contacting us.

I am sorry to hear that this product is not performing as expected. This product is covered by our Abpromise guarantee. If this is used according to the specification listed on the datasheet (on human in IHC-P,...

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