Recombinant
RabMAb

Anti-TrkA + TrkB + TrkC antibody [EPR18413] (ab189903)

Overview

  • Product name
    Anti-TrkA + TrkB + TrkC antibody [EPR18413]
  • Description
    Rabbit monoclonal [EPR18413] to TrkA + TrkB + TrkC
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TrkC aa 800 to the C-terminus (phospho Y820) (Cysteine residue). The exact sequence is proprietary. The original immunogen was phospho peptide but the antibody failed to specifically recognize the phospho site, recognizing phospho and nonphospho protein equally.
    Database link: Q16288-3

  • Positive control
    • WB: Human, mouse and rat brain lysates; SH-SY5Y whole cell lysate; HEK-293 whole cell lysate transfected with TrkA, TrkB and TrkC, respectively. IP: Mouse brain whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab189903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/40.
WB 1/1000. Detects a band of approximately 140,30 kDa (predicted molecular weight: 94 kDa).

Target

Images

  • All lanes : Anti-TrkA + TrkB + TrkC antibody [EPR18413] (ab189903) at 1/1000 dilution

    Lane 1 : Human brain lysate
    Lane 2 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
    Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique

    Predicted band size : 94 kDa
    Observed band size : 30,140 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    Blocking/Dilution buffer: 5% NFDM/TBST.

    TrkB is abundantly expressed in the central and peripheral nervous system. The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification.

  • All lanes : Anti-TrkA + TrkB + TrkC antibody [EPR18413] (ab189903) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Rat heart lysate
    Lane 7 : Rat kidney lysate
    Lane 8 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Developed using the ECL technique

    Predicted band size : 94 kDa
    Observed band size : 30,140 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    Blocking/Dilution buffer: 5% NFDM/TBST.

    TrkB is abundantly expressed in the central and peripheral nervous system. The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification.

  • All lanes : Anti-TrkA + TrkB + TrkC antibody [EPR18413] (ab189903) at 1/5000 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with empty vector (vector control)
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkA with His/Myc-tag
    Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with empty vector (vector control)
    Lane 4 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB with His/Myc-tag
    Lane 5 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with empty vector (vector control)
    Lane 6 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkC with His/Myc-tag

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 94 kDa

     

    Exposure times: Lane 1-2: 1 minute; Lane 3-6: 30 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • TrkC (phospho Y820) was immunoprecipitated from 1 mg of mouse brain whole cell lysate with ab189903 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab189903 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.

    Lane 1: Mouse brain whole cell lysate 10 µg (Input).

    Lane 2: ab189903 IP in mouse brain whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab189903 in mouse brain whole cell lysate.

    Exposure time: 5 seconds.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

     

References

ab189903 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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