Overview

  • Product nameAnti-TrkA antibody
    See all TrkA primary antibodies
  • Description
    Rabbit polyclonal to TrkA
  • SpecificityRat Trk A receptor (high affinity nerve growth factor receptor). The staining obtained with ab8871 in the brain and the spinal dorsal horn is as previously described in the literature.
  • Tested applicationsSuitable for: IHC-Fr, IP, WB, ICC, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Extracellular fragment from the rat Trk A receptor (amino acids 1-416)

  • Positive control
    • rat dorsal root ganglion, rat brain tissue (eg. supra-optic nucleus)
  • General notesConcentration may vary depending on the batch. Current batch concentration is 3.8mg/ml.

Properties

Applications

Our Abpromise guarantee covers the use of ab8871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/1000 - 1/10000.
IP Use at an assay dependent concentration. Use at a concentration of 2 - 5 µg/30µl Protein A sepharose per sample. Incubate for 2-3 hours at 4°C, then wash the Sepharose beads four times with lysis buffer. Elute sepharose-bound proteins in 2x Laemmli Sample buffer.
WB Use a concentration of 1 µg/ml.
ICC Use at an assay dependent concentration.
IHC-FoFr 1/3000.

Target

  • FunctionReceptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand, it can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.
    Isoform TrkA-III is resistant to NGF, constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade. Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed.
  • Tissue specificityIsoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors.
  • Involvement in diseaseCongenital insensitivity to pain with anhidrosis
    Chromosomal aberrations involving NTRK1 are found in papillary thyroid carcinomas (PTCs) (PubMed:2869410, PubMed:7565764, PubMed:1532241). Translocation t(1;3)(q21;q11) with TFG generates the TRKT3 (TRK-T3) transcript by fusing TFG to the 3'-end of NTRK1 (PubMed:7565764). A rearrangement with TPM3 generates the TRK transcript by fusing TPM3 to the 3'-end of NTRK1 (PubMed:2869410). An intrachromosomal rearrangement that links the protein kinase domain of NTRK1 to the 5'-end of the TPR gene forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the C-terminus of the NTRK1 protein (PubMed:1532241).
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 protein kinase domain.
  • DomainThe transmembrane domain mediates interaction with KIDINS220.
    The extracellular domain mediates interaction with NGFR.
  • Post-translational
    modifications
    Ligand-mediated autophosphorylation. Interaction with SQSTM1 is phosphotyrosine-dependent. Autophosphorylation at Tyr-496 mediates interaction and phosphorylation of SHC1.
    N-glycosylated (Probable). Isoform TrkA-I is N-glycosylated.
    Ubiquitinated. Undergoes polyubiquitination upon activation; regulated by NGFR. Ubiquitination regulates the internalization of the receptor.
  • Cellular localizationCell membrane. Early endosome membrane. Late endosome membrane. Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity).
  • Information by UniProt
  • Database links
  • Alternative names
    • gp140trk antibody
    • High affinity nerve growth factor receptor antibody
    • High affinity nerve growth factor receptor precursor antibody
    • MTC antibody
    • Neurotrophic tyrosine kinase receptor type 1 antibody
    • NTRK1 antibody
    • NTRK1_HUMAN antibody
    • Oncogene TRK antibody
    • p14-TrkA antibody
    • p140 TrkA antibody
    • p140-TrkA antibody
    • Slow nerve growth antibody
    • Trk A antibody
    • TRK antibody
    • Trk-A antibody
    • TRK1 antibody
    • TRK1-transforming tyrosine kinase protein antibody
    • Tropomyosin-related kinase A antibody
    • Tyrosine kinase receptor A antibody
    • Tyrosine kinase receptor antibody
    see all

Anti-TrkA antibody images

  • Immunofluorescent staining for TrkA obtained with TrkA antibody (ab8871) in rat DRG. Picture taken with X10 objective. Animals were intracardially perfused with 4% PFA. Tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT.  Protocol : IHC on 30um cryostat sections. Primary antibody ab8871 was used at 1/3000 and incubated overnight at room temperature. Secondary antibody, Alexa fluor 488 at 1/1000, incubated for 2 hours at room temperature.
  • All lanes : Anti-TrkA antibody (ab8871) at 1/1000 dilution

    Lane 1 : Whole cell lysate prepared from rat PC12 cells, un-treated.
    Lane 2 : Whole cell lysate prepared from rat PC12 cells, treated with 10 ng/ml NGF for 5 minutes.

    Lysates/proteins at 200000 cells per lane.

    Secondary
    HRP Goat anti-rabbit at 1/10000 dilution

    Observed band size : 110,140 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

    Image courtesy of an Abreview submitted by Joe Makkerh.

References for Anti-TrkA antibody (ab8871)

This product has been referenced in:
  • Wang TC  et al. Characterization of highly proliferative secondary tumor clusters along host blood vessels in malignant glioma. Mol Med Rep 12:6435-44 (2015). WB, IHC-Fr ; Rat . Read more (PubMed: 26299849) »
  • Thauerer B  et al. LAMTOR2-mediated modulation of NGF/MAPK activation kinetics during differentiation of PC12 cells. PLoS One 9:e95863 (2014). WB ; Rat . Read more (PubMed: 24752675) »

See all 8 Publications for this product

Product Wall

Application Immunocytochemistry
Sample Mouse Cultured Cells (dorsal root ganglion)
Specification dorsal root ganglion
Fixative Formaldehyde
Permeabilization Yes - cold methanol for 5 min
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Dr. Zunyi Wang

Verified customer

Submitted Feb 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (PC12)
Loading amount 200000 cells
Specification PC12
Treatment Lane 1 Untreated Lane 2: NGF 10 ng/ml, 5 minutes
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Joe Makkerh

Verified customer

Submitted Oct 01 2007

Thank you for your email and I'm very pleased to hear that the antibody worked well for you. We cannot provide you with any complimentary vials, but I do suggest that you submit an Abreview and in return we'll award you points that can be used in excha...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Rat brain, spinal cord, dorsal root ganglion)
Specification Rat brain, spinal cord, dorsal root ganglion
Fixative Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

Submitted Apr 12 2006

Thank you for your enquiry. The antibody comes in liquid form. It would depend on the lot ordered, but the last lot we shipped was 100 micrograms (3.8 mg/ml). The antibody is shipped in PBS (0.14 M Sodium Chloride; 0.003 M Potassium Chloride; 0.002 M P...

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The concentration is 2.0 mg/ml

The concentration will vary from lot to lot. The concentration of the recent lot we currently sell is 2.0 mg/ml. Regarding IHC on paraffin sections, originally this antibody has not been tested for this application. However, one of our customers repo...

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We have had no feedback on cross-reactivity with human and have not seen any published data that would confirm or deny it.

Thank you for your enquiry. All the information that we have regarding ab8871 is located on the online datasheet. I am sorry but we do not have an example of a Western blot for this antibody.

Many thanks for sending me your detailed protocol. I have taken a look at it and I have a few suggestions: 1) Only fix the specimins for 10 minutes in Zamboni's fixative. 2 hours may be too long, as the paraformaldehyde could cross-link the pro...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"