Recombinant
RabMAb

Anti-TrkA antibody [EPR1104(2)] (ab109010)

Overview

  • Product name
    Anti-TrkA antibody [EPR1104(2)]
    See all TrkA primary antibodies
  • Description
    Rabbit monoclonal [EPR1104(2)] to TrkA
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human TrkA.

  • Positive control
    • Human fetal brain and SH SY5Y lysates; Human brain tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 87 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand, it can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.
      Isoform TrkA-III is resistant to NGF, constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade. Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed.
    • Tissue specificity
      Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors.
    • Involvement in disease
      Congenital insensitivity to pain with anhidrosis
      Chromosomal aberrations involving NTRK1 are found in papillary thyroid carcinomas (PTCs) (PubMed:2869410, PubMed:7565764, PubMed:1532241). Translocation t(1;3)(q21;q11) with TFG generates the TRKT3 (TRK-T3) transcript by fusing TFG to the 3'-end of NTRK1 (PubMed:7565764). A rearrangement with TPM3 generates the TRK transcript by fusing TPM3 to the 3'-end of NTRK1 (PubMed:2869410). An intrachromosomal rearrangement that links the protein kinase domain of NTRK1 to the 5'-end of the TPR gene forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the C-terminus of the NTRK1 protein (PubMed:1532241).
    • Sequence similarities
      Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 2 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 protein kinase domain.
    • Domain
      The transmembrane domain mediates interaction with KIDINS220.
      The extracellular domain mediates interaction with NGFR.
    • Post-translational
      modifications
      Ligand-mediated autophosphorylation. Interaction with SQSTM1 is phosphotyrosine-dependent. Autophosphorylation at Tyr-496 mediates interaction and phosphorylation of SHC1.
      N-glycosylated (Probable). Isoform TrkA-I is N-glycosylated.
      Ubiquitinated. Undergoes polyubiquitination upon activation; regulated by NGFR. Ubiquitination regulates the internalization of the receptor.
    • Cellular localization
      Cell membrane. Early endosome membrane. Late endosome membrane. Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity).
    • Information by UniProt
    • Database links
    • Alternative names
      • gp140trk antibody
      • High affinity nerve growth factor receptor antibody
      • High affinity nerve growth factor receptor precursor antibody
      • MTC antibody
      • Neurotrophic tyrosine kinase receptor type 1 antibody
      • NTRK1 antibody
      • NTRK1_HUMAN antibody
      • Oncogene TRK antibody
      • p14-TrkA antibody
      • p140 TrkA antibody
      • p140-TrkA antibody
      • Slow nerve growth antibody
      • Trk A antibody
      • TRK antibody
      • Trk-A antibody
      • TRK1 antibody
      • TRK1-transforming tyrosine kinase protein antibody
      • Tropomyosin-related kinase A antibody
      • Tyrosine kinase receptor A antibody
      • Tyrosine kinase receptor antibody
      see all

    Images

    • All lanes : Anti-TrkA antibody [EPR1104(2)] (ab109010) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : SH SY5Y lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 87 kDa
    • ab109010 at 1/250 dilution staining TrkA in Human brain by Immunohistochemistry, Paraffin-embedded tissue.
    • ab109010 showing negative staining in Normal kidney tissue.

    • ab109010 showing negative staining in Normal liver tissue.

    • ab109010 showing negative staining in Normal tonsil tissue.

    • ab109010 showing negative staining in Normal colon tissue.

    References

    ab109010 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry
    Sample
    Mouse Cell (DRG neuron)
    Specification
    DRG neuron
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.2% Triton X-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted May 02 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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