• Product nameAnti-TrkA (phospho Y490) antibody
    See all TrkA primary antibodies
  • Description
    Rabbit polyclonal to TrkA (phospho Y490)
  • SpecificityThe antibody detects 140 kDa Trk A only when phosphorylated at Tyr490.
  • Tested applicationsSuitable for: ICC, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Available on request



Our Abpromise guarantee covers the use of ab1445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 10 - 20 µg/ml.
IP Use a concentration of 4 - 8 µg/ml.
WB Use a concentration of 1 µg/ml.


  • FunctionReceptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand, it can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.
    Isoform TrkA-III is resistant to NGF, constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade. Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed.
  • Tissue specificityIsoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors.
  • Involvement in diseaseCongenital insensitivity to pain with anhidrosis
    Chromosomal aberrations involving NTRK1 are found in papillary thyroid carcinomas (PTCs) (PubMed:2869410, PubMed:7565764, PubMed:1532241). Translocation t(1;3)(q21;q11) with TFG generates the TRKT3 (TRK-T3) transcript by fusing TFG to the 3'-end of NTRK1 (PubMed:7565764). A rearrangement with TPM3 generates the TRK transcript by fusing TPM3 to the 3'-end of NTRK1 (PubMed:2869410). An intrachromosomal rearrangement that links the protein kinase domain of NTRK1 to the 5'-end of the TPR gene forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the C-terminus of the NTRK1 protein (PubMed:1532241).
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 protein kinase domain.
  • DomainThe transmembrane domain mediates interaction with KIDINS220.
    The extracellular domain mediates interaction with NGFR.
  • Post-translational
    Ligand-mediated autophosphorylation. Interaction with SQSTM1 is phosphotyrosine-dependent. Autophosphorylation at Tyr-496 mediates interaction and phosphorylation of SHC1.
    N-glycosylated (Probable). Isoform TrkA-I is N-glycosylated.
    Ubiquitinated. Undergoes polyubiquitination upon activation; regulated by NGFR. Ubiquitination regulates the internalization of the receptor.
  • Cellular localizationCell membrane. Early endosome membrane. Late endosome membrane. Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity).
  • Information by UniProt
  • Database links
  • Alternative names
    • gp140trk antibody
    • High affinity nerve growth factor receptor antibody
    • High affinity nerve growth factor receptor precursor antibody
    • MTC antibody
    • Neurotrophic tyrosine kinase receptor type 1 antibody
    • NTRK1 antibody
    • NTRK1_HUMAN antibody
    • Oncogene TRK antibody
    • p140 TrkA antibody
    • p140-TrkA antibody
    • Slow nerve growth antibody
    • Trk A antibody
    • TRK antibody
    • Trk-A antibody
    • TRK1 antibody
    • TRK1-transforming tyrosine kinase protein antibody
    • Tropomyosin-related kinase A antibody
    • Tyrosine kinase receptor A antibody
    • Tyrosine kinase receptor antibody
    see all

References for Anti-TrkA (phospho Y490) antibody (ab1445)

This product has been referenced in:
  • Wiesner T  et al. Kinase fusions are frequent in Spitz tumours and spitzoid melanomas. Nat Commun 5:3116 (2014). Read more (PubMed: 24445538) »
  • Woronowicz A  et al. Trypanosome trans-sialidase mediates neuroprotection against oxidative stress, serum/glucose deprivation, and hypoxia-induced neurite retraction in Trk-expressing PC12 cells. Glycobiology 17:725-34 (2007). ICC/IF . Read more (PubMed: 17389653) »

See all 3 Publications for this product

Product Wall

Thank you for your enquiry and your prompt response.

Could you please confirm if you purchased this product via our distributor Sapphire and provide the Abcam Order number or the date of order. Once we got this information, we can place a new...

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Thank you for your response and for sharing your results.

Interestingly, there are two main bands on the blot (100, 150 kDa) and the intensity of the staining is higher with 300 nM of NGF than with 30 nM which is encouraging.


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Thank you for getting back to me.

I would suggest suing the blocking solution (5% BSA in TBS-T) for blocking the non-specific binding sites and for diluting the primary and the secondary antibodies as well.

Let me know how you are g...

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Thank you for your response. It may be worth running a blot with BSA as blocking agent if you still have some antibody left.

As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the p...

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Thank you for your enquiry regarding ab1445 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

Normally, for detection of phospho protein we woul...

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Thank you for contacting us with your questions last week, and I apologize for the delay getting back to you.

I have been in touch with the lab that produces these antibodies to ask for more information. There are several different isoforms o...

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Thank you for confirming how you wish to proceed. I am sorry that you do not wish to try the antibody again but I can understand your position. I am sorry that the antibody did not work as specified on the datasheet. As requested, I haveorganisedfora c...

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Thank you very much for providing that extra information it has indeed been very useful to understand the situation further.

I think having viewed your protocol there are a few things that I may be able to suggest to optimise which may improv...

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Thank you for providing that extra information. It has allowed me to understand much more fully the experience you have been having with anti-TrkA antibody (ab1445).

It is quite clear that you have been spending a lot of your time trying to o...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (hippocampus)
Loading amount 50 µg
Specification hippocampus
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted May 11 2011