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Synthetic peptide corresponding to Human TrkB. Surrounding amino acid 810 of human TrkB.
(Peptide available as
Our Abpromise guarantee covers the use of ab18987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 2 - 10 µg/ml.|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).Can be blocked with TrkB peptide (ab52216).|
|IHC-P||Use at an assay dependent concentration.|
ab18987 staining TrkB in rat spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/250 in blocking buffer) for 2 hours at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.
ab18987 staining TrkB in rat PC12 cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with methanol, permeabilized with 0.1% Saponin / PBS and blocked with 4% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (5 µg/ml in blocking buffer) for 14 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (ab150077) (1/100) was used as the secondary antibody.