Overview

  • Product nameAnti-TrkB antibody
    See all TrkB primary antibodies
  • Description
    Rabbit polyclonal to TrkB
  • SpecificityRecognizes rat TrkB, Mr 145kD, as well as an additional protein, Mr ~90kD, which is likely a TrkB degradation product.
  • Tested applicationsSuitable for: ICC/IF, IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    The entire extracellular domain (corresponding to residues 1 to 429) of rat TrkB.

  • Positive control
    • Mouse brain membrane protein preparation.

Properties

Applications

Our Abpromise guarantee covers the use of ab33655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 19923466
IHC-P Use at an assay dependent concentration. PubMed: 22646479
IP Use at an assay dependent concentration. PubMed: 23729812
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 145 kDa (predicted molecular weight: 92 kDa).

Target

  • FunctionReceptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
  • Tissue specificityIsoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Ligand-mediated auto-phosphorylation.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI848316 antibody
    • BDNF tropomyosine receptor kinase B antibody
    • BDNF/NT 3 growth factors receptor antibody
    • BDNF/NT-3 growth factors receptor antibody
    • Brain derived neurotrophic factor receptor antibody
    • C030027L06Rik antibody
    • EC 2.7.10.1 antibody
    • GP145 TrkB antibody
    • GP145-TrkB antibody
    • GP145-TrkB/GP95-TrkB antibody
    • GP95 TrkB antibody
    • Neurotrophic tyrosine kinase receptor type 2 antibody
    • Neurotrophin receptor tyrosine kinase type 2 antibody
    • NTRK 2 antibody
    • Ntrk2 antibody
    • NTRK2_HUMAN antibody
    • Obesity, hyperphagia, and developmental delay, included antibody
    • RATTRKB1 antibody
    • Tkrb antibody
    • Trk B antibody
    • Trk-B antibody
    • TRKB antibody
    • TrkB tyrosine kinase antibody
    • TRKB1 antibody
    • Tropomyosin related kinase B antibody
    • tyrosine kinase receptor B antibody
    • Tyrosine receptor kinase B antibody
    see all

Anti-TrkB antibody images

  • Immunohistochemical analysis of paraffin embedded mouse P29-RA lung section (10µm thick) labelling TrkB with ab33655 at 1:200 dilution. The secondary antibody was a peroxidase-labeled, polymer-conjugated goat anti-rabbit secondary antibody. Sections were stained with hematoxylin and eosin (H&E). Red arrow indicates bronchiolar epithelial cells, green arrow indicates type II pneumocyte and blue arrow indicates endothelial cells.

     

  • Anti-TrkB antibody (ab33655) at 1 µg/ml + Mouse brain membrane protein

    Predicted band size : 92 kDa

References for Anti-TrkB antibody (ab33655)

This product has been referenced in:
  • Balu DT  et al. Multiple risk pathways for schizophrenia converge in serine racemase knockout mice, a mouse model of NMDA receptor hypofunction. Proc Natl Acad Sci U S A 110:E2400-9 (2013). IP ; Mouse . Read more (PubMed: 23729812) »
  • Vohra PK  et al. TRPC3 regulates release of brain-derived neurotrophic factor from human airway smooth muscle. Biochim Biophys Acta 1833:2953-60 (2013). Read more (PubMed: 23899746) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Brain - Cerebellar granule cells - DIV3)
Loading amount 30 µg
Specification Brain - Cerebellar granule cells - DIV3
Treatment 100 ng/ml human BDNF for 6 minutes
Gel Running Conditions Reduced Denaturing (8% SDS PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (Brain - Cerebellar granule cells - DIV3)
Total protein in input 700 µg
Specification Brain - Cerebellar granule cells - DIV3
Treatment 100 ng/ml human BDNF for 6 minutes
Immuno-precipitation step Protein A
Username

Abcam user community

Verified customer

Submitted Mar 12 2013

Thank you for contacting us. Having compared the anti-phospho antibodies provided by Santa Cruz to those of Abcam (ab52191 and ab51187) they appear to be suitable for the same application in targeting the phosphorylated tyrosine-705 and -515 of...

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