Overview

  • Product nameAnti-TrkB antibody
    See all TrkB primary antibodies
  • Description
    Rabbit polyclonal to TrkB
  • SpecificityFrom experiments using similar antisera, it is anticipated that the reagent will block TrkB receptor binding of corresponding Nerve Growth factor ligand.
  • Tested applicationsSuitable for: ELISA, IHC-Frmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Extracellular domain of Mouse TrkB.

  • Positive control
    • Rat peripheral sensory ganglia.

Properties

Applications

Our Abpromise guarantee covers the use of ab43079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
IHC-Fr 1/3000.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionReceptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
    • Tissue specificityIsoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 2 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 LRRNT domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Ligand-mediated auto-phosphorylation.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI848316 antibody
      • BDNF tropomyosine receptor kinase B antibody
      • BDNF/NT 3 growth factors receptor antibody
      • BDNF/NT-3 growth factors receptor antibody
      • Brain derived neurotrophic factor receptor antibody
      • C030027L06Rik antibody
      • EC 2.7.10.1 antibody
      • GP145 TrkB antibody
      • GP145-TrkB antibody
      • GP145-TrkB/GP95-TrkB antibody
      • GP95 TrkB antibody
      • Neurotrophic tyrosine kinase receptor type 2 antibody
      • Neurotrophin receptor tyrosine kinase type 2 antibody
      • NTRK 2 antibody
      • Ntrk2 antibody
      • NTRK2_HUMAN antibody
      • Obesity, hyperphagia, and developmental delay, included antibody
      • RATTRKB1 antibody
      • Tkrb antibody
      • Trk B antibody
      • Trk-B antibody
      • TRKB antibody
      • TrkB tyrosine kinase antibody
      • TRKB1 antibody
      • Tropomyosin related kinase B antibody
      • tyrosine kinase receptor B antibody
      • Tyrosine receptor kinase B antibody
      see all

    Anti-TrkB antibody images

    • The cryostat section of the rat spinal cord was incubated in rabbit polyclonal antibodies to the extracellular domain of mouse TrkB at the dilution of 1/3000 overnight followed with a biotinylated secondary antibodies and ABC kit. The section was developed in diaminobenzidine solution and enhanced in the presence of nickel sulphate. Nerve fibres in the ventral horn are stained.

    References for Anti-TrkB antibody (ab43079)

    ab43079 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Rat Tissue sections (adult brain: cerebrum, hippocampus)
    Specification adult brain: cerebrum, hippocampus
    Fixative acetone-methanol (2:3)
    Permeabilization No
    Blocking step ProteinBlock (DakoCytomation) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 20°C
    Username

    Mr. Angelika Richter

    Verified customer

    Submitted Oct 25 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"