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Our Abpromise guarantee covers the use of ab6180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 18596175|
|IHC-Fr||Use at an assay dependent concentration.|
ab6180 staining rat lung tissue sections by IHC-P. Sections were fixed in acetone-methanol (3:2) and subjected to a heat mediated antigen retrieval step in citrate buffer (pH 6) prior to a 5 minute permeabilization in 0.5% TritonX-100 in PBS and blocking in 5% milk for 30 minutes at 20°C. The primary antibody was diluted 1/100 and incubated with sample for 1 hour at 20°C. A HRP conjugated donkey anti-rabbit antibody, diluted 1/50, was used as the secondary.