Overview

  • Product nameAnti-TrkB antibody
    See all TrkB primary antibodies
  • Description
    Rabbit polyclonal to TrkB
  • SpecificitySequence was chosen to avoid cross reactivity with trkA and trkC. Cross reactivity: Human and rat. Cross reactivity with other species has not been tested yet.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Synthetic peptide corresponding to Rat TrkB aa 23-36 (extracellular) conjugated to Keyhole Limpet Haemocyanin (KLH) (Glutaraldehyde).
    Sequence:

    AFPRLEPNSIDPENC

  • General notesThis antibody appears to have the same characteristics as those descried in Yan et al (1997). The immunising peptide sequence and conjugate are the same.

Properties

Applications

Our Abpromise guarantee covers the use of ab6180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration. PubMed: 18596175
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionReceptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
  • Tissue specificityIsoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Ligand-mediated auto-phosphorylation.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI848316 antibody
    • BDNF tropomyosine receptor kinase B antibody
    • BDNF/NT 3 growth factors receptor antibody
    • BDNF/NT-3 growth factors receptor antibody
    • Brain derived neurotrophic factor receptor antibody
    • C030027L06Rik antibody
    • EC 2.7.10.1 antibody
    • GP145 TrkB antibody
    • GP145-TrkB antibody
    • GP145-TrkB/GP95-TrkB antibody
    • GP95 TrkB antibody
    • Neurotrophic tyrosine kinase receptor type 2 antibody
    • Neurotrophin receptor tyrosine kinase type 2 antibody
    • NTRK 2 antibody
    • Ntrk2 antibody
    • NTRK2_HUMAN antibody
    • Obesity, hyperphagia, and developmental delay, included antibody
    • RATTRKB1 antibody
    • Tkrb antibody
    • Trk B antibody
    • Trk-B antibody
    • TRKB antibody
    • TrkB tyrosine kinase antibody
    • TRKB1 antibody
    • Tropomyosin related kinase B antibody
    • tyrosine kinase receptor B antibody
    • Tyrosine receptor kinase B antibody
    see all

Anti-TrkB antibody images

  • ab6180 staining rat lung tissue sections by IHC-P.  Sections were fixed in acetone-methanol (3:2) and subjected to a heat mediated antigen retrieval step in citrate buffer (pH 6) prior to a 5 minute permeabilization in 0.5% TritonX-100 in PBS and blocking in 5% milk for 30 minutes at 20°C.  The primary antibody was diluted 1/100 and incubated with sample for 1 hour at 20°C.  A HRP conjugated donkey anti-rabbit antibody, diluted 1/50, was used as the secondary.

    See Abreview

References for Anti-TrkB antibody (ab6180)

This product has been referenced in:
  • Sciesielski LK  et al. Enhanced pulmonary expression of the TrkB neurotrophin receptor in hypoxic rats is associated with increased acetylcholine-induced airway contractility. Acta Physiol (Oxf) 197:253-64 (2009). IHC-P ; Rat . Read more (PubMed: 19583705) »
  • Kuczewski N  et al. Backpropagating action potentials trigger dendritic release of BDNF during spontaneous network activity. J Neurosci 28:7013-23 (2008). ICC/IF ; Rat . Read more (PubMed: 18596175) »

See all 3 Publications for this product

Product Wall

Merci pour votre appel de hier. Le laboratoire m'avais répondu qu'ils ne connaissent pas non plus l'identité de cette bande. Je suis désolée.
Voici leur réponse:
"We know of no reason why a 20kD band should be present. While it is possible th...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Lung)
Specification Lung
Fixative acetone-methanol (3:2)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer, pH 6.0
Permeabilization Yes - 15 min 0.5%TritonX-100 in PBS
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Mr. Angelika Richter

Verified customer

Submitted Oct 11 2007

Thank you for your enquiry. This is a peptide found in the extra cellular domain, the on line data sheet has now bee updated to reflect this new information. If you need any further assistance then please do not hesitate to get back in touch with me.

Thank you for your enquiry. Unfortunately we do not have a blocking peptide specifically for use with ab6180. If you have any more questions, please contact us again.

We do not have an antibody to Tyrosine Receptor Kinase C. I suggest that you use the Abcam search engine to look for other potential suppliers of this antibody -- click on the "other companies" tab for non-Abcam results. However, I conducted a search f...

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As yet, this antibody has only been used for immunohistochemistry studies.

The tissue that we use as a positive control is the rat dorsal root ganglion. Using PFA containing picric acid as the fixative a population of neurons with medium to large diameter are stained. This population has been well described in the literature ...

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This antibody has not been used by us for Western blot. While we would expect that it should be useful for this technique, we cannot guarantee this.

PFA alone or with picric acid will both give good staining, although we prefer the addtion of picric acid. Pre-perfusion is with sodium nitrite (1% in phosphate buffer). In rat dorsal root ganglia, the neurons stained are the in the medium to large ran...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"