TrkB overexpression 293T lysate (whole cell) (ab94193)

Overview

  • Product nameTrkB overexpression 293T lysate (whole cell)
  • Description
    TrkB overexpression lysate
  • General notesab94193 is a 293T cell transfected lysate in which Human TrkB has been transiently over-expressed using a pCMV-TrkB plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1. Tissue specificity: Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 2 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain. Contains 1 protein kinase domain. PTM: Ligand-mediated auto-phosphorylation.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

TrkB overexpression 293T lysate (whole cell) images

  • ab94193 at 15µg/lane on an SDS-PAGE gel
  • All lanes : Anti-TrkB antibody (ab55749) at 1/500 dilution

    Lane 1 : TrkB overexpression 293T lysate (whole cell) (ab94193)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for TrkB overexpression 293T lysate (whole cell) (ab94193)

ab94193 has not yet been referenced specifically in any publications.

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