The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIs unsuitable for WB.
FunctionReceptor for neurotrophin-3 (NT-3). This is a tyrosine-protein kinase receptor. Known substrates for the trk receptors are SHC1, PI-3 kinase, and PLCG1. The different isoforms do not have identical signaling properties.
Tissue specificityWidely expressed but mainly in nervous tissue. Isoform B is expressed at higher levels in adult brain than in fetal brain.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 2 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 protein kinase domain.
ETS related protein neurotrophic receptor tyrosine kinase fusion antibody
ETS related protein neurotrophic receptor tyrosine kinase fusion protein antibody
ETV6 NTRK3 fusion antibody
GP145 TrkC antibody
Neurotrophic tyrosine kinase receptor type 3 antibody
Neurotrophin 3 receptor antibody
NT 3 growth factor receptor antibody
NT 3 growth factor receptor precursor antibody
NT 3 receptor antibody
NT-3 growth factor receptor antibody
TRK C antibody
TrkC tyrosine kinase antibody
Tyrosine kinase receptor C antibody
Anti-TrkC antibody images
Immunocytochemistry/ Immunofluorescence - Anti-TrkC antibody (ab43078)This image is courtesy of an anonymous abreview.
Immunocytochemistry/ Immunofluorescence analysis of human U2OS cells labeling TrkC with ab43078 at 1/250. Cells were fixed in formaldehyde and permeabilized with 3% milk powder + 0.1% Triton X-100 in PBS. Blocking of the cells was done with 3% milk for 1 hour at 22°C, followed by staining with ab43078 at 1/250 in 3% milk powder+0.1% Triton X-100 in PBS for 1 hour at 22°C. A FITC conjugated goat anti-rabbit secondary antibody was used at 1/400 dilution.
Figure shows expression and cellular locaization of endogenous TrkC in U2OS cells. DAPI nuclear stain is also shown in adjacent picture.