Overview

  • Product name
  • Description
    Rabbit polyclonal to TrkC
  • Specificity
    From experiments using similar antisera, it is anticipated that the reagent will block TrkC receptor binding of corresponding Nerve Growth factor ligand.
  • Tested applications
    Suitable for: ICC/IF, IHC-Frmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Extracellular domain of Human TrkC.

  • Positive control
    • Rat peripheral sensory ganglia.

Properties

Applications

Our Abpromise guarantee covers the use of ab43078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
IHC-Fr 1/3000.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Receptor for neurotrophin-3 (NT-3). This is a tyrosine-protein kinase receptor. Known substrates for the trk receptors are SHC1, PI-3 kinase, and PLCG1. The different isoforms do not have identical signaling properties.
    • Tissue specificity
      Widely expressed but mainly in nervous tissue. Isoform B is expressed at higher levels in adult brain than in fetal brain.
    • Sequence similarities
      Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 2 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Ligand-mediated auto-phosphorylation.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • EC 2.7.10.1 antibody
      • ETS related protein neurotrophic receptor tyrosine kinase fusion antibody
      • ETS related protein neurotrophic receptor tyrosine kinase fusion protein antibody
      • ETV6 NTRK3 fusion antibody
      • GP145 TrkC antibody
      • gp145(trkC) antibody
      • GP145-TrkC antibody
      • GP145TrkC antibody
      • Neurotrophic tyrosine kinase receptor type 3 antibody
      • Neurotrophin 3 receptor antibody
      • NT 3 growth factor receptor antibody
      • NT 3 growth factor receptor precursor antibody
      • NT 3 receptor antibody
      • NT-3 growth factor receptor antibody
      • Ntrk3 antibody
      • NTRK3_HUMAN antibody
      • OTTHUMP00000192915 antibody
      • TRK C antibody
      • Trk-C antibody
      • TRKC antibody
      • TrkC tyrosine kinase antibody
      • Tyrosine kinase receptor C antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence analysis of human U2OS cells labeling TrkC with ab43078 at 1/250. Cells were fixed in formaldehyde and permeabilized with 3% milk powder + 0.1% Triton X-100 in PBS. Blocking of the cells was done with 3% milk for 1 hour at 22°C, followed by staining with ab43078 at 1/250 in 3% milk powder+0.1% Triton X-100 in PBS for 1 hour at 22°C. A FITC conjugated goat anti-rabbit secondary antibody was used at 1/400 dilution.

      Figure shows expression and cellular locaization of endogenous TrkC in U2OS cells. DAPI nuclear stain is also shown in adjacent picture.

      See Abreview

    References

    This product has been referenced in:
    • Zong H  et al. A Hyperactive Signalosome in Acute Myeloid Leukemia Drives Addiction to a Tumor-Specific Hsp90 Species. Cell Rep 13:2159-73 (2015). Read more (PubMed: 26628369) »
    • Cesca F  et al. Kidins220/ARMS mediates the integration of the neurotrophin and VEGF pathways in the vascular and nervous systems. Cell Death Differ 19:194-208 (2012). Read more (PubMed: 22048155) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Endogenous TrkC expression in U2OS)
    Specification
    Endogenous TrkC expression in U2OS
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 3% milk powder+0.1% Triton X-100 in PBS
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 19 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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