Overview

  • Product name
    Anti-TRMT12 antibody
  • Description
    Rabbit polyclonal to TRMT12
  • Specificity
    ab113103 is predicted to not cross-react with other TRMT protein family members. At least two isoforms of TRMT12 are known to exist, ab113013 will detect both isoforms.
  • Tested applications
    Suitable for: WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide containing a 19 amino acid sequence from the N terminal region of Human TRMT12 (Q53H54).

  • Positive control
    • K562 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: 99.98% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab113103 is purified by affinity chromatography via a peptide column.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab113103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 50 kDa.
ICC Use a concentration of 10 µg/ml.

Target

  • Function
    Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA.
  • Pathway
    tRNA modification; wybutosine-tRNA(Phe) biosynthesis.
  • Sequence similarities
    Belongs to the RNA methyltransferase trmD family. TYW2 subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha amino alpha carboxypropyl transferase TYW2 antibody
    • FLJ20772 antibody
    • Homolog of yeast tRNA methyltransferase antibody
    • Radical S-adenosyl methionine and flavodoxin domain-containing protein 2 antibody
    • TRM 12 antibody
    • TRM12 antibody
    • TRMT 12 antibody
    • TRMT12 antibody
    • tRNA methyltransferase 12 antibody
    • tRNA methyltransferase 12 homolog antibody
    • tRNA wybutosine-synthesizing protein 2 homolog antibody
    • tRNA-yW-synthesizing protein 2 antibody
    • TYW2 antibody
    • TYW2_HUMAN antibody
    see all

Images

  • All lanes : Anti-TRMT12 antibody (ab113103) at 0.5 µg/ml

    Lane 1 : K562 cell lysate
    Lane 2 : K562 cell lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 50 kDa
  • Immunocytochemical analysis of K562 cells, staining TRMT12 with ab113103 at 10 µg/ml.

References

ab113103 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab113103.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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