Overview

  • Product nameAnti-TRMT2B antibody
    See all TRMT2B primary antibodies
  • Description
    Mouse polyclonal to TRMT2B
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human TRMT2B. Full length human FLJ12687 protein (NP_079193, 1 a.a. ~ 504 a.a).

  • Positive control
    • Human stomach tissue lysate. FLJ12687 transfected lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: Whole serum
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 57 kDa).

Target

  • FunctionProbable S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the formation of 5-methyl-uridine at position 54 (m5U54) in all tRNA. May also have a role in tRNA stabilization or maturation.
  • Sequence similaritiesBelongs to the methyltransferase superfamily. RNA M5U methyltransferase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chromosome X open reading frame 34 antibody
    • CXorf34 antibody
    • dJ341D10.3 antibody
    • FLJ12687 antibody
    • Putative methyltransferase CXorf34 antibody
    • TRM2 homolog antibody
    • TRM2 tRNA methyltransferase 2 homolog B (S. cerevisiae) antibody
    • TRM2 tRNA methyltransferase 2 homolog B antibody
    • TRM2_HUMAN antibody
    • TRMT2B antibody
    • tRNA (uracil 5 )methyltransferase homolog antibody
    • tRNA (uracil(54)-C(5))-methyltransferase homolog antibody
    • tRNA (uracil-5-)-methyltransferase homolog antibody
    • tRNA methyltransferase 2 homolog B antibody
    see all

Anti-TRMT2B antibody images

  • Anti-TRMT2B antibody (ab69053) at 1/500 dilution + Human stomach tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 57 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-TRMT2B antibody (ab69053) at 1/500 dilution

    Lane 1 : FLJ12687 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 57 kDa

References for Anti-TRMT2B antibody (ab69053)

ab69053 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"