Overview

  • Product nameAnti-TRMT5 antibody
    See all TRMT5 primary antibodies
  • Description
    Rabbit polyclonal to TRMT5
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 460-509 (EMLCITFQIP ASVLYKNQTR NPENHEDPPL KRQRTAEAFS DEKTQIVSNT) of human TRMT5 (NP_065861).

  • Positive control
    • MCF7 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:62500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionSpecifically methylates guanosine-37 in various tRNAs. Not dependent on the nature of the nucleoside 5' of the target nucleoside.
  • Sequence similaritiesBelongs to the TRM5 / TYW2 family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1393 antibody
    • M1G methyltransferase antibody
    • M1G-methyltransferase antibody
    • TRM5 antibody
    • TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae) antibody
    • TRMT5 antibody
    • TRMT5_HUMAN antibody
    • tRNA (guanine-N(1)-)-methyltransferase antibody
    • tRNA [GM37] methyltransferase antibody
    • tRNA methyltransferase 5 antibody
    • tRNA N1G37 methyltransferase antibody
    see all

Anti-TRMT5 antibody images

  • Anti-TRMT5 antibody (ab83379) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58 kDa
    Observed band size : 67 kDa (why is the actual band size different from the predicted?)

References for Anti-TRMT5 antibody (ab83379)

ab83379 has not yet been referenced specifically in any publications.

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