Overview

  • Product nameAnti-TRNT1 antibody
    See all TRNT1 primary antibodies
  • Description
    Rabbit polyclonal to TRNT1
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: INNRGEKHGT ITARLHEENF EITTLRIDVT TDGRHAEVEF TTDWQKDAER, corresponding to N terminal amino acids 72-121 of Human TRNT1

  • Positive control
    • Fetal brain lysate; human kidney tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab50895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 16 µg/ml.

Target

  • RelevanceTRNT1 belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. It adds and repairs the conserved 3'-CCA sequence necessary for the attachment of amino acids to the 3' terminus of tRNA molecules, using CTP and ATP as substrates.
  • Cellular localizationMitochondrial
  • Database links
  • Alternative names
    • AA408384 antibody
    • C76540 antibody
    • CCA adding antibody
    • CCA1 antibody
    • CGI 47 antibody
    • EC 2.7.7.25 antibody
    • Mitochondrial CCA adding tRNA nucleotidyltransferase antibody
    • Mitochondrial tRNA nucleotidyl transferase, CCA adding antibody
    • mt CCA adding enzyme antibody
    • mt Trat antibody
    • mt tRNA adenylyltransferase antibody
    • mt tRNA CCA diphosphorylase antibody
    • mt tRNA CCA pyrophosphorylase antibody
    • MtCCA antibody
    • MTO2 antibody
    • TRMU antibody
    • tRNA nucleotidyl transferase antibody
    • tRNA nucleotidyl transferase CCA adding 1 antibody
    see all

Anti-TRNT1 antibody images

  • Anti-TRNT1 antibody (ab50895) at 2.5 µg/ml + Fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 50 kDa
  • ab50895 at 16µg/ml positively labelling epithelial cells of renal tubule (indicated by arrows) in paraffin-embedded human kidney tissue. Magnification 400x

References for Anti-TRNT1 antibody (ab50895)

ab50895 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"