Anti-TROP2 antibody [162-46.2] (ab79976)
Key features and details
- Mouse monoclonal [162-46.2] to TROP2
- Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-TROP2 antibody [162-46.2]
See all TROP2 primary antibodies -
Description
Mouse monoclonal [162-46.2] to TROP2 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human TROP2. Human choriocarcinoma cell line BeWo.
-
Positive control
- IHC-P: Human placenta tissue. ICC/IF: JEG3 and MCF7 cells. Flow Cyt (Intra): JEG3 and MCF7 cells.
-
General notes
Isotype: IgG1 (Igh-4a allotype)
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
162-46.2 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
Use 0.1-1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-P |
Use at an assay dependent concentration.
|
|
ICC/IF |
Use a concentration of 5 µg/ml.
|
Notes |
---|
Flow Cyt (Intra)
Use 0.1-1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 5 µg/ml. |
Target
-
Function
May function as a growth factor receptor. -
Tissue specificity
Placenta, pancreatic carcinoma cell lines. -
Involvement in disease
Defects in TACSTD2 are the cause of gelatinous drop-like corneal dystrophy (GDLD) [MIM:204870]; also known as lattice corneal dystrophy type III. GDLD is an autosomal recessive disorder characterized by grayish corneal amyloid deposits that cause severe visual impairment. -
Sequence similarities
Belongs to the EPCAM family.
Contains 1 thyroglobulin type-1 domain. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 4070 Human
- Omim: 137290 Human
- SwissProt: P09758 Human
- Unigene: 23582 Human
-
Alternative names
- Cell surface glycoprotein Trop 2 antibody
- Cell surface glycoprotein Trop-2 antibody
- Cell surface glycoprotein Trop2 antibody
see all
Images
-
Overlay histogram showing JEG3 cells stained with ab79976 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab79976, 0.1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150117) at 1/2000 dilution for 30 min at 22°C.
Isotype control antibody (black line) was mouse IgG1 [15-6E10A7] (monoclonal) (ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
This antibody gave a positive signal in JEG3 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
-
ab79976 staining TROP2 in MCF7 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab79976 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
-
Overlay histogram showing MCF7 cells stained with ab79976 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79976, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed MCF7 cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
-
ab79976 staining TROP2 in human 10 week placenta by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Anti-mouse peroxidase IgG used as secondary antibody. Note staining of cytotrophoblast (CT) and syncytiotrophoblast (ST).
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab79976 has been referenced in 4 publications.
- Grünherz L et al. Osteoidosis leads to altered differentiation and function of osteoclasts. J Cell Mol Med 24:5665-5674 (2020). PubMed: 32283567
- Im H et al. Digital diffraction analysis enables low-cost molecular diagnostics on a smartphone. Proc Natl Acad Sci U S A 112:5613-8 (2015). PubMed: 25870273
- Viswanatha R et al. Interactome analysis reveals ezrin can adopt multiple conformational states. J Biol Chem 288:35437-51 (2013). ICC/IF ; Human . PubMed: 24151071
- Lipinski M et al. Human trophoblast cell-surface antigens defined by monoclonal antibodies. Proc Natl Acad Sci U S A 78:5147-50 (1981). PubMed: 7029529