Anti-TROP2 antibody (ab65006)

Overview

  • Product name
  • Description
    Rabbit polyclonal to TROP2
  • Specificity
    This antibody detects endogenous levels of total TROP2 protein.
  • Tested applications
    Suitable for: IHC-P, WB, ELISA, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide corresponding to Human TROP2 (internal sequence).

  • Positive control
    • WB: A549 and NIH-3T3 cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
ELISA 1/5000.
ICC Use at an assay dependent concentration.

Target

  • Function
    May function as a growth factor receptor.
  • Tissue specificity
    Placenta, pancreatic carcinoma cell lines.
  • Involvement in disease
    Defects in TACSTD2 are the cause of gelatinous drop-like corneal dystrophy (GDLD) [MIM:204870]; also known as lattice corneal dystrophy type III. GDLD is an autosomal recessive disorder characterized by grayish corneal amyloid deposits that cause severe visual impairment.
  • Sequence similarities
    Belongs to the EPCAM family.
    Contains 1 thyroglobulin type-1 domain.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell surface glycoprotein Trop 2 antibody
    • Cell surface glycoprotein Trop-2 antibody
    • Cell surface glycoprotein Trop2 antibody
    • Epithelial glycoprotein 1 antibody
    • GA733 1 antibody
    • GA7331 antibody
    • M1S 1 antibody
    • M1S1 antibody
    • Membrane component chromosome 1 surface marker 1 antibody
    • Pancreatic carcinoma marker protein GA733 1 antibody
    • Pancreatic carcinoma marker protein GA733-1 antibody
    • Pancreatic carcinoma marker protein GA7331 antibody
    • TACD 2 antibody
    • TACD2_HUMAN antibody
    • TACSTD 2 antibody
    • Tacstd2 antibody
    • Trop 2 antibody
    • Trop2 antibody
    • Tumor associated calcium signal transducer 2 precursor antibody
    • Tumor-associated calcium signal transducer 2 antibody
    see all

Anti-TROP2 antibody images

  • All lanes : Anti-TROP2 antibody (ab65006) at 1/500 dilution

    Lane 1 : Extracts from A549 cells
    Lane 2 : Extracts from NIH-3T3 cells
    Lane 3 : Extracts from NIH-3T3 cells with immunising peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 37 kDa
    Observed band size : 37 kDa
  • IHC image of ab65006 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab65006, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-TROP2 antibody (ab65006)

ab65006 has not yet been referenced specifically in any publications.

Product Wall

Filter by Application

Filter by Species

Filter by Ratings

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cell (Prostate)
Permeabilization
Yes - Trixton X-100
Specification
Prostate
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Methanol
Username

Dr. Olayinka Oloko

Verified customer

Submitted Jun 17 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Prostate)
Gel Running Conditions
Non-reduced Denaturing (10%gel)
Loading amount
25 µg
Specification
Prostate
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 4°C
Username

Dr. Olayinka Oloko

Verified customer

Submitted Apr 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up