This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Anti-Trophoblast specific protein alpha antibody (ab104401)

Overview

  • Product name
    Anti-Trophoblast specific protein alpha antibody
  • Description
    Rabbit polyclonal to Trophoblast specific protein alpha

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Trophoblast specific protein alpha.

  • Positive control
    • This antibody gave a positive signal in Mouse trophoblast tissue lysate as well as the following whole cell lysates: MEF1; NIH3T3; STO.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
ELISA Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Relevance
    Trophoblast specific protein alpha is a marker of spongiotrophoblasts.
  • Database links
  • Alternative names
    • Tb1 antibody
    • Tpbp antibody
    • Tpbpa antibody
    • Tpbpa trophoblast specific protein alpha antibody
    • Trophoblast specific protein alpha antibody
    • Trophoblast specific protein antibody
    see all

Anti-Trophoblast specific protein alpha antibody images

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • All lanes : Anti-Trophoblast specific protein alpha antibody (ab104401) at 1 µg/ml

    Lane 1 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Trophoblast Stem Cells (Mouse) Whole Cell Lysate
    Lane 4 : STO (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 14 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa,255 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 14 minutes
  • ICC/IF image of ab104401 stained MEF1 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96899, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab104401, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MEF1 cells at 1µg/ml.

References for Anti-Trophoblast specific protein alpha antibody (ab104401)

This product has been referenced in:
  • Nakamura K  et al. Increase in complement iC3b is associated with anti-inflammatory cytokine expression during late pregnancy in mice. PLoS One 12:e0178442 (2017). IHC-Fr ; Mouse . Read more (PubMed: 28542608) »
  • King A  et al. Tumor-homing peptides as tools for targeted delivery of payloads to the placenta. Sci Adv 2:e1600349 (2016). IF . Read more (PubMed: 27386551) »

See all 3 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Placenta)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Placenta
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde
Username

Dr. Athilakshmi Kannan

Verified customer

Submitted Aug 13 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (E13.5 placenta)
Permeabilization
Yes - 5% goat serum and 0.05% Triton X-100 in PBS
Specification
E13.5 placenta
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Dr. Paul Delgado Olguin

Verified customer

Submitted May 13 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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