• Product nameAnti-Tropoelastin antibody
  • Description
    Rabbit polyclonal to Tropoelastin
  • Tested applicationsSuitable for: ICC, IHC-Fr, IHC-P, ELISA, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide (containing sequences from exons 6-17)(Mouse).

  • Positive control
    • In Western Blot, this antibody gave a positive signal in human heart tissue lysate.



Our Abpromise guarantee covers the use of ab21600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/50 - 1/100.
IHC-Fr 1/50 - 1/100.
IHC-P Use at an assay dependent concentration.
ELISA 1/1000 - 1/2000.
WB 1/500. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).
IP 1/100 - 1/200.


  • FunctionMajor structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.
  • Tissue specificityExpressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.
  • Involvement in diseaseDefects in ELN are a cause of autosomal dominant cutis laxa (ADCL) [MIM:123700]. Cutis laxa is a rare connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. The skin changes are often accompanied by extracutaneous manifestations, including pulmonary emphysema, bladder diverticula, pulmonary artery stenosis and pyloric stenosis.
    Defects in ELN are the cause of supravalvular aortic stenosis (SVAS) [MIM:185500]. SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome.
    Note=ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • Sequence similaritiesBelongs to the elastin family.
  • Post-translational
    Elastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
    Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
  • Cellular localizationSecreted > extracellular space > extracellular matrix. Extracellular matrix of elastic fibers.
  • Information by UniProt
  • Database links
  • Alternative names
    • Elastin antibody
    • ELN antibody
    • ELN_HUMAN antibody
    • SVAS antibody
    • Tropoelastin antibody
    see all

Anti-Tropoelastin antibody images

  • Anti-Tropoelastin antibody (ab21600) at 1/500 dilution + Human heart tissue lysate - total protein (ab29431) at 10 µg

    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 72 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

References for Anti-Tropoelastin antibody (ab21600)

This product has been referenced in:
  • Liu S  et al. Dynamic expression of chymotrypsin-like elastase 1 over the course of murine lung development. Am J Physiol Lung Cell Mol Physiol 306:L1104-16 (2014). Read more (PubMed: 24793170) »
  • Cheng J  et al. Pharmacological inhibitor of notch signaling stabilizes the progression of small abdominal aortic aneurysm in a mouse model. J Am Heart Assoc 3:e001064 (2014). IHC-P ; Mouse . Read more (PubMed: 25349182) »

See all 6 Publications for this product

Product Wall

Thank you for your enquiry.

I can confirm that ab21600 antibody is sold as whole antiserum. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datashe...

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Thank you for your message and for providing this further information.

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I look forward to hearing from you with details of how you would like to proceed.

Thank you for your inquiry.

The lab said that they would think ab21607 & ab21610 would recognize the precursor of elastin, aka tropoelastin, but this hasn't been specifically tested.

I think I found an antibody that would work for...

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Thank you for contacting us.

Neither ab21605 or ab21600 have been tested in plants, and we guarantee our products work in the tested species and applications stated on the datasheet.

ab21605, which has been discontinued, was generated...

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Thank you for your inquiry.

Indeed, there is the risk that this antibody would not work on the human tropoelastin expressed in tobacco plant due to differential posttranslational modifications. However I think that the chances are high that t...

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Thank you for enquiry. I have been in touch with the source of this antibody and they have come back with a few comments. "Please can you tell me whether you intend to distinguish tropoelastin from insoluble elastin by IHC in in intact tissues....

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Thank you for your phone call. The difference between these two antibodies are the immunogens that were used to produce the antibodies. Ab21600 was raised against a peptide containing sequences from exons 6-17 (mouse). Ab21601 was raised against a pept...

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