Anti-Tropomodulin 1 antibody [1F2] (ab124034)

Overview

  • Product nameAnti-Tropomodulin 1 antibody [1F2]
    See all Tropomodulin 1 primary antibodies
  • Description
    Mouse monoclonal [1F2] to Tropomodulin 1
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human Tropomodulin 1 produced in HEK293 cells (NP_003266).

  • Positive control
    • HEK293T cell lysate transfected with pCMV6-ENTRY Tropomodulin 1 cDNA; MCF7 cell extract; Human pancreas carcinoma tissue; COS7 cells transiently transfected with pCMV6-ENTRY Tropomodulin 1; HEK293T cells transfected with a Tropomodulin 1 overexpress plasmid
  • General notesDilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

Properties

Applications

Our Abpromise guarantee covers the use of ab124034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 41 kDa.
IHC-P 1/50.
Flow Cyt 1/100. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionBlocks the elongation and depolymerization of the actin filaments at the pointed end. The Tmod/TM complex contributes to the formation of the short actin protofilament, which in turn defines the geometry of the membrane skeleton. May play an important role in regulating the organization of actin filaments by preferentially binding to a specific tropomyosin isoform at its N-terminus.
  • Tissue specificityHighly expressed in the erythrocyte, heart and skeletal muscle.
  • Sequence similaritiesBelongs to the tropomodulin family.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • D9S57E antibody
    • E Tmod antibody
    • E tropomodulin antibody
    • E-Tmod antibody
    • Erythrocyte tropomodulin antibody
    • ETMOD antibody
    • MGC124125 antibody
    • MGC124126 antibody
    • OTTHUMP00000021743 antibody
    • TMOD antibody
    • Tmod1 antibody
    • TMOD1 protein antibody
    • TMOD1_HUMAN antibody
    • Tropomodulin 1 antibody
    • Tropomodulin-1 antibody
    see all

Anti-Tropomodulin 1 antibody [1F2] images

  • All lanes : Anti-Tropomodulin 1 antibody [1F2] (ab124034) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Tropomodulin 1 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 41 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC201134)
  • All lanes : Anti-Tropomodulin 1 antibody [1F2] (ab124034) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 41 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC201134)
  • ab124034, at 1/50 dilution, staining Tropomodulin 1 in paraffin-embedded Human pancreas carcinoma tissue by Immunohistochemistry.
  • ab124034, at 1/100 dilution, staining Tropomodulin 1 in COS7 cells transiently transfected with pCMV6-ENTRY Tropomodulin 1 by Immunofluorescence.
  • ab124034, at 1/100 dilution, staining Tropomodulin 1 in HEK293T cells transfected with a Tropomodulin 1 overexpress plasmid (Red) or empty vector control plasmid (Blue) by Flow cytometry.

References for Anti-Tropomodulin 1 antibody [1F2] (ab124034)

ab124034 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"