Anti-Tropomodulin 2 antibody [EPR6353] (ab124833)

Overview

  • Product nameAnti-Tropomodulin 2 antibody [EPR6353]
    See all Tropomodulin 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR6353] to Tropomodulin 2
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N-terminus of Human Tropomodulin 2.

  • Positive control
    • Fetal brain and SH-SY5Y cell lysates; Human brain tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab124833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • RelevanceTropomodulin is the actin-capping protein for the slow growing end of filamentous actin. Tropomodulin 2 is a neuronal specific isoform which is the major binding protein to brain tropomyosin in rat.
    • Cellular localizationCytoplasm; cytoskeleton
    • Database links
    • Alternative names
      • Tropomodulin 2 antibody
      • MGC39481 antibody
      • N Tmod antibody
      • Neuronal tropomodulin antibody
      • NTMOD antibody
      • TMOD2 antibody
      • tropomodulin 2 (neuronal) antibody
      • tropomodulin 2 neuronal antibody
      see all

    Anti-Tropomodulin 2 antibody [EPR6353] images

    • All lanes : Anti-Tropomodulin 2 antibody [EPR6353] (ab124833) at 1/10000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 40 kDa
      Observed band size : 40 kDa
    • ab124833, at 1/250 dilution, staining Tropomodulin 2 in paraffin-embedded Human brain tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Tropomodulin 2 antibody [EPR6353] (ab124833)

    ab124833 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Murine Neuroblastome N1E-115)
    Specification Murine Neuroblastome N1E-115
    Fixative Formaldehyde
    Permeabilization Yes - 0.1% Triton, 10min
    Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 03 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (N1E-115 mouse neuroblastoma)
    Loading amount 20 µg
    Specification N1E-115 mouse neuroblastoma
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 25 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"