The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 21245055
Use a concentration of 1.25 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 33 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
FunctionBinds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
Tissue specificityDetected in primary breast cancer tissues but undetectable in normal breast tissues in Sudanese patients. Isoform 1 is expressed in adult and fetal skeletal muscle and cardiac tissues, with higher expression levels in the cardiac tissues. Isoform 10 is expressed in adult and fetal cardiac tissues, but not in skeletal muscle.
Involvement in diseaseDefects in TPM1 are the cause of cardiomyopathy familial hypertrophic type 3 (CMH3) [MIM:115196]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Defects in TPM1 are the cause of cardiomyopathy dilated type 1Y (CMD1Y) [MIM:611878]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence similaritiesBelongs to the tropomyosin family.
DomainThe molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tropomyosin 1 (alpha) antibody (ab55915)This image was kindly supplied by Dr Jinqing Li by Abreview
ab55915 at a 1/250 dilution staining Tropomyosin 1 (alpha) in mouse heart tissue sections by Immunohistochemistry (paraffin embedded) incubated for 16 hours at +4°C. Fixed in formaldehyde, heat mediated antigen retrieval performed using citrate buffer. Blocked using 10% serum for 20 minutes at 20°C. Secondary used undiluted polyclonal Goat anti-rabbit IgG conjugated to Hilyte FluorTM 488.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) anlysis of Human Muscle lysate tissue labelling Tropomyosin 1 (alpha) with ab55915 at 8µg/ml. Positively labelled skeletal muscle cells are indicated with arrows. Magnification: 400X
References for Anti-Tropomyosin 1 (alpha) antibody (ab55915)
This product has been referenced in:
Bortolini MA et al. Expression of genes encoding smooth muscle contractile proteins in vaginal tissue of women with and without pelvic organ prolapse. Neurourol Urodyn31:109-14 (2012).
Read more (PubMed: 22038928) »
Li J et al. Up-regulation of p27kip1 contributes to Nrf2-mediated protection against angiotensin II-induced cardiac hypertrophy. Cardiovasc Res90:315-24 (2011).
Read more (PubMed: 21245055) »