Overview

  • Product nameAnti-Tropomyosin 1 (alpha) antibody
    See all Tropomyosin 1 (alpha) primary antibodies
  • Description
    Rabbit polyclonal to Tropomyosin 1 (alpha)
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Dog, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Pig, Zebrafish
  • Immunogen

    Synthetic peptide: KQLEDELVSL QKKLKGTEDE LDKYSEALKD AQEKLELAEK KATDAEADVA , corresponding to amino acids 37-86 of Human Tropomyosin 1 (alpha)

  • Positive control
    • HepG2 cell lysate Human muscle: skeletal muscle cells

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab55915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21245055
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 33 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionBinds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
  • Tissue specificityDetected in primary breast cancer tissues but undetectable in normal breast tissues in Sudanese patients. Isoform 1 is expressed in adult and fetal skeletal muscle and cardiac tissues, with higher expression levels in the cardiac tissues. Isoform 10 is expressed in adult and fetal cardiac tissues, but not in skeletal muscle.
  • Involvement in diseaseDefects in TPM1 are the cause of cardiomyopathy familial hypertrophic type 3 (CMH3) [MIM:115196]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TPM1 are the cause of cardiomyopathy dilated type 1Y (CMD1Y) [MIM:611878]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similaritiesBelongs to the tropomyosin family.
  • DomainThe molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA986836 antibody
    • AI854628 antibody
    • Alpha tropomyosin antibody
    • alpha-TM antibody
    • Alpha-tropomyosin antibody
    • C15orf13 antibody
    • cardiomyopathy, hypertrophic 3 antibody
    • CMD1Y antibody
    • CMH3 antibody
    • HTM alpha antibody
    • HTM-alpha antibody
    • OTTHUMP00000163688 antibody
    • sarcomeric tropomyosin kappa antibody
    • TM2 antibody
    • Tmpa antibody
    • TMSA antibody
    • Tpm-1 antibody
    • TPM1 antibody
    • TPM1_HUMAN antibody
    • tropomyosin 1 (alpha) antibody
    • tropomyosin 1 (alpha) isoform 1 antibody
    • tropomyosin 1 (alpha) isoform 2 antibody
    • tropomyosin 1 (alpha) isoform 3 antibody
    • tropomyosin 1 (alpha) isoform 4 antibody
    • tropomyosin 1 (alpha) isoform 5 antibody
    • tropomyosin 1 (alpha) isoform 6 antibody
    • tropomyosin 1 (alpha) isoform 7 antibody
    • Tropomyosin 1 antibody
    • Tropomyosin alpha 1 chain antibody
    • Tropomyosin alpha-1 chain antibody
    • Tropomyosin, skeletal muscle alpha antibody
    • Tropomyosin-1 antibody
    see all

Anti-Tropomyosin 1 (alpha) antibody images

  • Anti-Tropomyosin 1 (alpha) antibody (ab55915) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 33 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • ab55915 at a 1/250 dilution staining Tropomyosin 1 (alpha) in mouse heart tissue sections by Immunohistochemistry (paraffin embedded) incubated for 16 hours at +4°C. Fixed in formaldehyde, heat mediated antigen retrieval performed using citrate buffer. Blocked using 10% serum for 20 minutes at 20°C. Secondary used undiluted polyclonal Goat anti-rabbit IgG conjugated to Hilyte FluorTM 488.

    See Abreview

  • All lanes : Anti-Tropomyosin 1 (alpha) antibody (ab55915) at 1.25 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate with blocking peptide at 1 µg/ml

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 33 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) anlysis of Human Muscle lysate tissue labelling Tropomyosin 1 (alpha) with ab55915 at 8µg/ml. Positively labelled skeletal muscle cells are indicated with arrows. Magnification: 400X

References for Anti-Tropomyosin 1 (alpha) antibody (ab55915)

This product has been referenced in:
  • Bortolini MA  et al. Expression of genes encoding smooth muscle contractile proteins in vaginal tissue of women with and without pelvic organ prolapse. Neurourol Urodyn 31:109-14 (2012). IHC-P ; Human . Read more (PubMed: 22038928) »
  • Li J  et al. Up-regulation of p27kip1 contributes to Nrf2-mediated protection against angiotensin II-induced cardiac hypertrophy. Cardiovasc Res 90:315-24 (2011). ICC/IF ; Rat . Read more (PubMed: 21245055) »

See all 5 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Sample Zebrafish Cell (Muscle)
Specification Muscle
Permeabilization Yes - 0.3% Triton
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 03 2015

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing (12%)
Sample Dog Cell lysate - whole cell (heart)
Specification heart
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Glen Pyle

Verified customer

Submitted Dec 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (heart)
Specification heart
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Dr. Jinqing Li

Verified customer

Submitted Dec 23 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"