Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)

Overview

  • Product nameAnti-Tropomyosin 1 (alpha) antibody [EPR5158]
    See all Tropomyosin 1 (alpha) primary antibodies
  • Description
    Rabbit monoclonal [EPR5158] to Tropomyosin 1 (alpha)
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • WB: lysate from Human heart or skeletal muscle; HT-1080 cell lysate. IHC-P: Human muscle tissue (skeletal, heart and smooth).
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC or IP.
  • Target

    • FunctionBinds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
    • Tissue specificityDetected in primary breast cancer tissues but undetectable in normal breast tissues in Sudanese patients. Isoform 1 is expressed in adult and fetal skeletal muscle and cardiac tissues, with higher expression levels in the cardiac tissues. Isoform 10 is expressed in adult and fetal cardiac tissues, but not in skeletal muscle.
    • Involvement in diseaseDefects in TPM1 are the cause of cardiomyopathy familial hypertrophic type 3 (CMH3) [MIM:115196]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
      Defects in TPM1 are the cause of cardiomyopathy dilated type 1Y (CMD1Y) [MIM:611878]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    • Sequence similaritiesBelongs to the tropomyosin family.
    • DomainThe molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.
    • Cellular localizationCytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • AA986836 antibody
      • AI854628 antibody
      • Alpha tropomyosin antibody
      • alpha-TM antibody
      • Alpha-tropomyosin antibody
      • C15orf13 antibody
      • cardiomyopathy, hypertrophic 3 antibody
      • CMD1Y antibody
      • CMH3 antibody
      • HTM alpha antibody
      • HTM-alpha antibody
      • OTTHUMP00000163688 antibody
      • sarcomeric tropomyosin kappa antibody
      • TM2 antibody
      • Tmpa antibody
      • TMSA antibody
      • Tpm-1 antibody
      • TPM1 antibody
      • TPM1_HUMAN antibody
      • tropomyosin 1 (alpha) antibody
      • tropomyosin 1 (alpha) isoform 1 antibody
      • tropomyosin 1 (alpha) isoform 2 antibody
      • tropomyosin 1 (alpha) isoform 3 antibody
      • tropomyosin 1 (alpha) isoform 4 antibody
      • tropomyosin 1 (alpha) isoform 5 antibody
      • tropomyosin 1 (alpha) isoform 6 antibody
      • tropomyosin 1 (alpha) isoform 7 antibody
      • Tropomyosin 1 antibody
      • Tropomyosin alpha 1 chain antibody
      • Tropomyosin alpha-1 chain antibody
      • Tropomyosin, skeletal muscle alpha antibody
      • Tropomyosin-1 antibody
      see all

    Anti-Tropomyosin 1 (alpha) antibody [EPR5158] images

    • All lanes : Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505) at 1/1000 dilution

      Lane 1 : Human heart lysate
      Lane 2 : Human skeletal muscle lysate
      Lane 3 : HT-1080 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 33 kDa
    • Immunohistochemical analysis of paraffin-embedded Human heart muscle tissue using ab109505 at 1/500
    • Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue using ab109505 at 1/500
    • Immunohistochemical analysis of paraffin-embedded Human smooth muscle tissue using ab109505 at 1/500

    References for Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)

    ab109505 has not yet been referenced specifically in any publications.

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