Overview

  • Product name
    Anti-Tropomyosin 2 antibody
    See all Tropomyosin 2 primary antibodies
  • Description
    Rabbit polyclonal to Tropomyosin 2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Cow, Pig
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 254 of Human Tropomyosin 2 (UniProt ID: P07951_2).

  • Positive control
    • HeLa whole cell lysate for WB and SW480 Xenograph for IHC-P.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 33 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function
    Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. The non-muscle isoform may have a role in agonist-mediated receptor internalization.
  • Tissue specificity
    Present in primary breast cancer tissue, absent from normal breast tissue.
  • Involvement in disease
    Nemaline myopathy 4 (NEM4) [MIM:609285]: A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 4 presents from infancy to childhood with hypotonia and moderate-to-severe proximal weakness with minimal or no progression. Major motor milestones are delayed but independent ambulation is usually achieved, although a wheelchair may be needed in later life. Note=The disease is caused by mutations affecting the gene represented in this entry.
    Arthrogryposis, distal, 1A (DA1A) [MIM:108120]: A form of distal arthrogryposis, a disease characterized by congenital joint contractures that mainly involve two or more distal parts of the limbs, in the absence of a primary neurological or muscle disease. Distal arthrogryposis type 1 is characterized largely by camptodactyly and clubfoot. Hypoplasia and/or absence of some interphalangeal creases is common. The shoulders and hips are less frequently affected. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similarities
    Belongs to the tropomyosin family.
  • Domain
    The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.
  • Post-translational
    modifications
    Phosphorylated on Ser-61 by PIK3CG. Phosphorylation on Ser-61 is required for ADRB2 internalization.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha tropomyosin antibody
    • AMCD1 antibody
    • Arthrogryposis multiplex congenital distal type 1 antibody
    • Beta tropomyosin antibody
    • Beta-tropomyosin antibody
    • Cytoskeletal tropomyosin TM30 antibody
    • DA1 antibody
    • DA2B antibody
    • epididymis secretory protein Li 273 antibody
    • FLJ41118 antibody
    • Heat stable cytoskeletal protein 30 kDa antibody
    • HEL-S-273 antibody
    • hscp30 antibody
    • HTM alpha antibody
    • hTM5 antibody
    • MGC14582 antibody
    • MGC3261 antibody
    • MGC72094 antibody
    • NEM1 antibody
    • NEM4 antibody
    • Nemaline myopathy type 4 antibody
    • OK/SW cl.5 antibody
    • Sarcomeric tropomyosin kappa antibody
    • TM 5 antibody
    • TM3 antibody
    • TM30 antibody
    • TM30nm antibody
    • TMSA antibody
    • TMSB antibody
    • TPM 1 antibody
    • TPM 3 antibody
    • TPM1 alpha antibody
    • TPM1 kappa antibody
    • TPM2 antibody
    • TPM2_HUMAN antibody
    • TRK antibody
    • Tropomyosin 1 alpha antibody
    • Tropomyosin 1 alpha chain antibody
    • Tropomyosin 1 alpha chain isoform 6 antibody
    • Tropomyosin 2 (beta) antibody
    • Tropomyosin 2 antibody
    • Tropomyosin 3 antibody
    • Tropomyosin alpha 3 chain antibody
    • Tropomyosin alpha striated muscle isoform antibody
    • Tropomyosin beta chain antibody
    • Tropomyosin gamma antibody
    • Tropomyosin skeletal muscle beta antibody
    • Tropomyosin-2 antibody
    see all

Images

  • Anti-Tropomyosin 2 antibody (ab96073) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 33 kDa
  • ab96073, at 1/100 dilution, staining Tropomyosin 2 in SW480 xenograft tissue by Immunohistochemistry. Formalin-fixed, paraffin-embedded section.

References

This product has been referenced in:
  • Azimzadeh O  et al. A dose-dependent perturbation in cardiac energy metabolism is linked to radiation-induced ischemic heart disease in Mayak nuclear workers. Oncotarget 8:9067-9078 (2017). WB . Read more (PubMed: 27391067) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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