Overview

  • Product name
    Anti-Tropomyosin 3 antibody
    See all Tropomyosin 3 primary antibodies
  • Description
    Rabbit polyclonal to Tropomyosin 3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Recombinant full length protein corresponding to Human Tropomyosin 3 aa 1-285.
    Sequence:

    MMEAIKKKMQ MLKLDKENAL DRAEQAEAEQ KQAEERSKQL EDELAAMQKK LKGTEDELDK YSEALKDAQE KLELAEKKAA DAEAEVASLN RRIQLVEEEL DRAQERLATA LQKLEEAEKA ADESERGMKV IENRALKDEE KMELQEIQLK EAKHIAEEAD RKYEEVARKL VIIEGDLERT EERAELAESK CSELEEELKN VTNNLKSLEA QAEKYSQKED KYEEEIKILT DKLKEAETRA EFAERSVAKL EKTIDDLEDE LYAQKLKYKA ISEELDHALN DMTSI


    Database link: P06753

  • Positive control
    • 293T cell and A431 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab180813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/500 - 1/2000. Predicted molecular weight: 33 kDa.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
  • Involvement in disease
    Defects in TPM3 are the cause of nemaline myopathy type 1 (NEM1) [MIM:609284]. A form of nemaline myopathy with autosomal dominant or recessive inheritance. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Autosomal dominant nemaline myopathy type 1 is characterized by a moderate phenotype with onset between birth and early second decade of life. Weakness is diffuse and symmetric with slow progression often with need for a wheelchair in adulthood. The autosomal recessive form has onset at birth with moderate-to-severe hypotonia and diffuse weakness. In the most severe cases, death can occur before 2 years. Less severe cases have delayed major motor milestones, and these patients may walk, but often need a wheelchair before 10 years.
    Defects in TPM3 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TPM3 is found in thyroid papillary carcinomas. A rearrangement with NTRK1 generates the TRK fusion transcript by fusing the amino end of isoform 2 of TPM3 to the 3'-end of NTRK1.
  • Sequence similarities
    Belongs to the tropomyosin family.
  • Domain
    The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha tropomyosin 3 antibody
    • Alpha tropomyosin slow skeletal antibody
    • CFTD antibody
    • Cytoskeletal tropomyosin TM30 antibody
    • FLJ41118 antibody
    • gamma TM antibody
    • Gamma tropomyosin antibody
    • Gamma-tropomyosin antibody
    • Heat stable cytoskeletal protein 30 kDa antibody
    • hscp30 antibody
    • hTM30nm antibody
    • hTM5 antibody
    • hTMnm antibody
    • MGC102590 antibody
    • MGC14582 antibody
    • MGC3261 antibody
    • MGC72094 antibody
    • NEM1 antibody
    • OK/SW-cl.5 antibody
    • OTTHUMP00000034019 antibody
    • OTTHUMP00000034171 antibody
    • OTTHUMP00000034172 antibody
    • TM 5 antibody
    • TM-5 antibody
    • TM3 antibody
    • TM30 antibody
    • TM30nm antibody
    • TM5 antibody
    • Tm5NM antibody
    • Tpm 5 antibody
    • TPM3 antibody
    • TPM3/NTRK1 FUSION GENE, INCLUDED antibody
    • TPM3_HUMAN antibody
    • Tpm5 antibody
    • TPMsk3 antibody
    • TRK antibody
    • TRK ONCOGENE, INCLUDED antibody
    • Trop 5 antibody
    • Tropomyosin 3 antibody
    • Tropomyosin 3 gamma antibody
    • Tropomyosin 5 antibody
    • Tropomyosin alpha 3 chain antibody
    • Tropomyosin alpha-3 chain antibody
    • Tropomyosin gamma antibody
    • Tropomyosin-3 antibody
    • Tropomyosin-5 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Tropomyosin 3 with ab180813 at 1/200. Magnification: 200x.
  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling TPM3 with ab180813 at a dilution of 1/50. Nuclear DNA was labelled with DAPI (shown in blue).

  • All lanes : Anti-Tropomyosin 3 antibody (ab180813) at 1/500 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : A431 cell lysate

    Predicted band size: 33 kDa

References

ab180813 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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