Overview

  • Product nameAnti-Tropomyosin antibody
    See all Tropomyosin primary antibodies
  • Description
    Rabbit polyclonal to Tropomyosin
  • SpecificityRabbit anti-Tropomyosin specifically stains tropomyosin in cell cultures using indirect immunofluorescent labeling. The staining of stress fibers shows a typical banded pattern. In immunoblotting, anti-Tropomyosin specifically stains the two tropomyosin bands.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Chicken
  • Immunogen

    Recombinant full length protein (Chicken).

  • Positive control
    • Total tissue extract.
  • General notesFor continuous use, store at 2-8 °C for up to one month. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: Whole serum
  • PurityWhole antiserum
  • Purification notesProduct is supplied as liquid whole antiserum. The antiserum has been treated to remove lipoproteins.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesIF: 1/40 (determined by indirect immunofluorescent labeling of cultured chicken fibroblasts).
    WB: 1/100 (determined by indirect immunoblotting using total tissue extract).

    Rabbit anti-Tropomyosin may be used for visualization of tropomyosin in various tissues and cell cultures. The product reacts best on cultured chicken fibroblasts.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceTropomyosin is a dimeric coiled coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F actin filaments, and the lack of proline and tryptophan.
    • Cellular localizationCytoplasmic
    • Database links
    • Alternative names
      • Alpha tropomyosin antibody
      • Beta tropomyosin antibody
      • TM30nm antibody
      • TPM1 antibody
      • TPM2 antibody
      • TPM3 antibody
      • Tropomyosin 1 alpha antibody
      • Tropomyosin 1 antibody
      • Tropomyosin 2 antibody
      • Tropomyosin 2 beta antibody
      • Tropomyosin 3 antibody
      see all

    References for Anti-Tropomyosin antibody (ab11190)

    ab11190 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab11190.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"