The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution. Detection limit for ab76640 is approximately 3 ng/ml as a capture antibody.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 33 kDa.
This antibody has only been tested in WB against the recombinant protein used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceTropomyosin is a dimeric coiled coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F actin filaments, and the lack of proline and tryptophan.