Overview

  • Product nameAnti-Tropomyosin antibody
    See all Tropomyosin primary antibodies
  • Description
    Mouse monoclonal to Tropomyosin
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity

    Predicted to work with: Human
  • Immunogen

    Recombinant full length human Tropomyosin protein (AAH11776) with a tag

  • Positive control
    • Recombinant full length Tropomyosin protein (immunogen).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Use at an assay dependent dilution. Detection limit for ab76640 is approximately 3 ng/ml as a capture antibody.

    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 33 kDa.
    This antibody has only been tested in WB against the recombinant protein used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

  • Target

    • RelevanceTropomyosin is a dimeric coiled coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F actin filaments, and the lack of proline and tryptophan.
    • Cellular localizationCytoplasmic
    • Database links
    • Alternative names
      • Alpha tropomyosin antibody
      • Beta tropomyosin antibody
      • TM30nm antibody
      • TPM1 antibody
      • TPM2 antibody
      • TPM3 antibody
      • Tropomyosin 1 alpha antibody
      • Tropomyosin 1 antibody
      • Tropomyosin 2 antibody
      • Tropomyosin 2 beta antibody
      • Tropomyosin 3 antibody
      see all

    Anti-Tropomyosin antibody images

    • Anti-Tropomyosin antibody (ab76640) at 5 µg/ml + immunogen at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 33 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)

    References for Anti-Tropomyosin antibody (ab76640)

    ab76640 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76640.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"