Overview

  • Product nameAnti-Tropomyosin antibody [CH1]
    See all Tropomyosin primary antibodies
  • Description
    Mouse monoclonal [CH1] to Tropomyosin
  • SpecificityThis antibody is specific for human striated muscle forms of tropomyosin, including cardiac alpha tropomyosin and skeletal muscle forms of tropomyosin. This rod-shaped protein is associated with contractile tissue, where it regulates the Ca 2+ -dependent interaction between actin and myosin. This antibody reacts with human striated muscle forms of tropomyosin. It also cross-reacts with chicken cardiac muscle. This antibody does not cross-react with smooth muscle or non-muscle isoforms of tropomyosin.
  • Tested applicationsSuitable for: IHC-FoFr, IHC-Fr, IP, IHC-P, RIA, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Chicken
  • Immunogen

    Purified chicken muscle tropomyosin.

  • Positive control
    • Human tongue, heart and chicken heart

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 20mM Tris, 150mM Sodium chloride
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone numberCH1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Prolonged fixation in buffered formalin may destroy the epitope.
RIA Use at an assay dependent concentration.
WB 1/50 - 1/100.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

Anti-Tropomyosin antibody [CH1] images

  • Anti-Tropomyosin antibody [CH1] (ab7786) at 1/200 dilution + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
  • Overlay histogram showing HeLa cells stained with ab7786 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7786, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-Tropomyosin antibody [CH1] (ab7786)

This product has been referenced in:
  • Yogev O  et al. eIF4EBP3L acts as a gatekeeper of TORC1 in activity-dependent muscle growth by specifically regulating Mef2ca translational initiation. PLoS Biol 11:e1001679 (2013). Read more (PubMed: 24143132) »
  • Agrawal PB  et al. Normal myofibrillar development followed by progressive sarcomeric disruption with actin accumulations in a mouse Cfl2 knockout demonstrates requirement of cofilin-2 for muscle maintenance. Hum Mol Genet 21:2341-56 (2012). Mouse . Read more (PubMed: 22343409) »

See all 3 Publications for this product

Product Wall

Thank you for your inquiry.

Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.

We aim to provide as much information as possible to our customers, so I am so...

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Both ab7786 and ab7785 are mouse monoclonals to chicken Tropomyosin. Both have been shown to cross react with rat. ab7786 has been shown to recognise human cardiac alpha tropomyosin and chicken cardiac muscle, and we therefore recommend this for your p...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"