Overview

  • Product name
    Anti-Tropomyosin antibody [MAC 141]
    See all Tropomyosin primary antibodies
  • Description
    Rat monoclonal [MAC 141] to Tropomyosin
  • Specificity
    Antibody reacts with tropomyosin in Lethocerus and Drosophila flight and non-flight muscle and with tropomyosin in flight muscles of all other insect species tested. Antibody reacts with Troponin H (TnH) in Lethocerus flight muscle, the 33 and 34 isoforms of TnH in Drosophila and proteins of TnH molecular weight in flight muscles of other Diptera and Lepidoptera. Antibody also reacts with tropomyosin in muscles of Crustacea and Caenorhabditis (nematode).
  • Tested applications
    Suitable for: WB, IHC-Fr, Electron Microscopy, ICC/IF, IHC - Wholemountmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster, Waterbug
    Predicted to work with: Caenorhabditis elegans, A wide range of other invertebrates
  • Immunogen

    Troponin-tropomyosin complex purified from Lethocerus indicus (Waterbug) flight muscle.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    MAC 141
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.
IHC-Fr Use at an assay dependent concentration. PubMed: 19272319
Electron Microscopy Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC - Wholemount Use at an assay dependent concentration. PubMed: 22841645

Target

  • Relevance
    Tropomyosin is a dimeric coiled coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F actin filaments, and the lack of proline and tryptophan.
  • Cellular localization
    Cytoplasmic
  • Alternative names
    • Alpha tropomyosin antibody
    • Beta tropomyosin antibody
    • TM30nm antibody
    • TPM1 antibody
    • TPM2 antibody
    • TPM3 antibody
    • Tropomyosin 1 alpha antibody
    • Tropomyosin 1 antibody
    • Tropomyosin 2 antibody
    • Tropomyosin 2 beta antibody
    • Tropomyosin 3 antibody
    see all

Anti-Tropomyosin antibody [MAC 141] images

  • Immunohistochemical analysis of wholemount Drosohopila melanogaster embryo, staining Tropomyosin with ab50567 at 1/1000 dilution.
  • Immunohistochemical analysis of wholemount Drosohopila melanogaster embryo, staining Tropomyosin with ab50567 at 1/1000 dilution.

References for Anti-Tropomyosin antibody [MAC 141] (ab50567)

This product has been referenced in:
  • Cabezas-Cruz A  et al. Anaplasma phagocytophilum Infection Subverts Carbohydrate Metabolic Pathways in the Tick Vector, Ixodes scapularis. Front Cell Infect Microbiol 7:23 (2017). ICC/IF . Read more (PubMed: 28229048) »
  • Auld AL & Folker ES Nucleus-dependent sarcomere assembly is mediated by the LINC complex. Mol Biol Cell 27:2351-9 (2016). Read more (PubMed: 27307582) »

See all 12 Publications for this product

Product Wall

Thank you for your enquiry. I have been informed by the source of this product of the following information: " In 1993 Dr Matthew Colloff (Dept Bacteriology, Stobhill General Hospital, Glasgow) said that MAC 141 reacts with prawn tropomyosin. He ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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