Overview

  • Product nameAnti-Tropomyosin antibody [TM-311]
    See all Tropomyosin primary antibodies
  • Description
    Mouse monoclonal [TM-311] to Tropomyosin
  • SpecificityThis antibody is specific for the 36K and 39K bands (alpha isoform at 39 kDa and beta isoform at 36 kDa )of chicken gizzard tropomyosin. It is not specific for the alpha form alone. This antibody stains human fibroblast cells, and it cross-reacts with chicken fibroblast and L8 rat muscle cells.
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Human
  • Immunogen

    Purified chicken gizzard tropomyosin.

  • Positive control
    • Chicken or human fibroblasts

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberTM-311
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/100 - 1/200. Detects a band of approximately 49-59 kDa (predicted molecular weight: 33 kDa).

Target

Anti-Tropomyosin antibody [TM-311] images

  • Anti-Tropomyosin antibody [TM-311] (ab7785) at 1/200 dilution + Human heart tissue lysate - total protein (ab29431) at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 33 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

References for Anti-Tropomyosin antibody [TM-311] (ab7785)

This product has been referenced in:
  • Ito A  et al. Induction of functional tissue-engineered skeletal muscle constructs by defined electrical stimulation. Sci Rep 4:4781 (2014). WB ; Mouse . Read more (PubMed: 24759171) »
  • Yamashiro S  et al. Differential actin-regulatory activities of Tropomodulin1 and Tropomodulin3 with diverse tropomyosin and actin isoforms. J Biol Chem 289:11616-29 (2014). WB ; Rabbit . Read more (PubMed: 24644292) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Myometrium)
Loading amount 20 µg
Specification Myometrium
Gel Running Conditions Reduced Denaturing (gel 12%,)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jan 14 2011

Thank you for your phone call. The originator of ab7785 provided me with the following information regarding this antibody. The molecular weight of tropomyosin is 70 kDa. The alpha isomer is 36 kDa and the beta isomer is 35 kDa. In electrophoresis...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"