SpecificityAntibody reacts with Troponin H (TnH) in Lethocerus flight muscle, the 34 isoform of TnH in Drosophila and proteins of TnH molecular weight in flight muscle of all other insect species tested. Antibody also reacts with zeelin 2 in Lethocerus and glutathione-S-transferase-2 kin Drosophila. Antibody reacts with Drosophila BiP (hsc72).
Tested applicationsSuitable for:
ICC/IF, Electron Microscopy, WBmore details
Drosophila melanogaster, Waterbug
Troponin-tropomyosin complex purified from Lethocerus indicus (Waterbug)flight muscle
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesElectron Microscopy: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceTroponin H (TnH) is a tropomyosin isoform found in insect indirect flight muscle. It has a C-terminal extension that connects to the myosin cross-bridge. Two isoforms of TnH exist in Drosophila, at 33 and 34 kDa.
References for Anti-Troponin H antibody [MAC 143] (ab50571)
This product has been referenced in:
Kulke M et al. Kettin, a major source of myofibrillar stiffness in Drosophila indirect flight muscle. J Cell Biol154:1045-57 (2001).
Read more (PubMed: 11535621) »
Clayton JD et al. Interaction of troponin-H and glutathione S-transferase-2 in the indirect flight muscles of Drosophila melanogaster. J Muscle Res Cell Motil19:117-27 (1998).
Read more (PubMed: 9536439) »