Recombinant
RabMAb

Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] (ab184554)

Overview

  • Product name
    Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)]
    See all Troponin I fast skeletal muscle primary antibodies
  • Description
    Rabbit monoclonal [EPR14927(B)] to Troponin I fast skeletal muscle
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Troponin I fast skeletal muscle aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: P48788

  • Positive control
    • Human skeletal muscle, mouse muscle and rat muscle lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR14927(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/20.
WB 1/10000 - 1/50000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

Target

  • Function
    Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Involvement in disease
    Defects in TNNI2 are a cause of distal arthrogryposis type 2B (DA2B) [MIM:601680]; also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin.
  • Sequence similarities
    Belongs to the troponin I family.
  • Information by UniProt
  • Database links
  • Alternative names
    • AMCD 2B antibody
    • AMCD2B antibody
    • DA 2B antibody
    • DA2B antibody
    • fast skeletal muscle antibody
    • Fast twitch skeletal muscle troponin I antibody
    • fast-twitch isoform antibody
    • FSSV antibody
    • fsTnI antibody
    • TNNI 2 antibody
    • Tnni2 antibody
    • TNNI2_HUMAN antibody
    • tro antibody
    • Troponin I antibody
    • Troponin I fast skeletal muscle antibody
    • Troponin I fast twitch 2 antibody
    • Troponin I fast twitch isoform antibody
    • Troponin I fast twitch skeletal muscle isoform antibody
    • Troponin I skeletal fast antibody
    • Troponin I type 2 (skeletal fast) antibody
    • Troponin I type 2 antibody
    see all

Images

  • Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] (ab184554) at 1/20000 dilution + Human skeletal muscle lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

  • All lanes : Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] (ab184554) at 1/20000 dilution

    Lane 1 : Mouse muscle lysate
    Lane 2 : Rat muscle lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

  • Western blot analysis of Troponin I fast skeletal muscle in Human skeletal muscle lysate immunoprecipitated using ab184554 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Human skeletal muscle lysate.

    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

References

ab184554 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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