Anti-Troponin I fast + slow skeletal muscle antibody [12F10] (ab110132)
- Datasheet
- Specific References
- Protocols
Overview
- Product nameAnti-Troponin I fast + slow skeletal muscle antibody [12F10]
See all Troponin I fast + slow skeletal muscle primary antibodies - DescriptionMouse monoclonal [12F10] to Troponin I fast + slow skeletal muscle
- SpecificityCross-reactivity with Cardiac Troponin I is less than 0.05%.
- Tested applicationsSuitable for: WB, IHC-Pmore details
- Species reactivityReacts with: Mouse
Predicted to work with: Human
- Immunogen
Mouse Troponin I fast + slow skeletal muscle.
- Positive control
- Human skeletal muscle tissue.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99.9% PBS -
Concentration information loading... - PurityProtein A purified
- ClonalityMonoclonal
- Clone number12F10
- IsotypeIgG2b
- Research areas
Associated products
- Compatible Secondaries
- Positive Controls
Applications
Our Abpromise guarantee covers the use of ab110132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 21 kDa. | |
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
- RelevanceTroponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue.
- Cellular localizationCytoplasmic and Nuclear
-
Database links
- Entrez Gene: 7135 Human
- Entrez Gene: 7136 Human
- Entrez Gene: 21952 Mouse
- Entrez Gene: 21953 Mouse
- Omim: 191042 Human
- Omim: 191043 Human
- SwissProt: P19237 Human
- SwissProt: P48788 Human
see all -
Alternative names
- AMCD2B antibody
- DA2B antibody
- DKFZp451O223 antibody
see all
Anti-Troponin I fast + slow skeletal muscle antibody [12F10] images
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Troponin I fast + slow skeletal muscle antibody [12F10] (ab110132)](http://a.abcam.com/ps/datasheet/images/110/ab110132/Troponin-I-fast-slow-skeletal-muscle-Primary-antibodies-ab110132-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Troponin I fast + slow skeletal muscle antibody [12F10] (ab110132)ab110132 at 10 µg/ml staining Troponin I fast + slow skeletal muscle in Formalin-Fixed, Paraffin-Embedded Human skeletal muscle tissue by Immunohistochemistry. Detection utilised biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
References for Anti-Troponin I fast + slow skeletal muscle antibody [12F10] (ab110132)
ab110132 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"