Anti-Troponin I fast + slow skeletal muscle antibody [12F10] (ab110132)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Troponin I fast + slow skeletal muscle antibody [12F10]
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DescriptionMouse monoclonal [12F10] to Troponin I fast + slow skeletal muscle
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SpecificityCross-reactivity with Cardiac Troponin I is less than 0.05%.
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Mouse
Predicted to work with: Human
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Immunogen
Mouse Troponin I fast + slow skeletal muscle.
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Positive control
- Human skeletal muscle tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99.9% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone number12F10
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IsotypeIgG2b
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab110132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 21 kDa. | |
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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RelevanceTroponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue.
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Cellular localizationCytoplasmic and Nuclear
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Database links
- Entrez Gene: 7135 Human
- Entrez Gene: 7136 Human
- Entrez Gene: 21952 Mouse
- Entrez Gene: 21953 Mouse
- Omim: 191042 Human
- Omim: 191043 Human
- SwissProt: P19237 Human
- SwissProt: P48788 Human
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Alternative names
- AMCD2B antibody
- DA2B antibody
- DKFZp451O223 antibody
see all
Anti-Troponin I fast + slow skeletal muscle antibody [12F10] images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Troponin I fast + slow skeletal muscle antibody [12F10] (ab110132)](http://a.abcam.com/ps/datasheet/images/110/ab110132/Troponin-I-fast-slow-skeletal-muscle-Primary-antibodies-ab110132-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Troponin I fast + slow skeletal muscle antibody [12F10] (ab110132)ab110132 at 10 µg/ml staining Troponin I fast + slow skeletal muscle in Formalin-Fixed, Paraffin-Embedded Human skeletal muscle tissue by Immunohistochemistry. Detection utilised biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
References for Anti-Troponin I fast + slow skeletal muscle antibody [12F10] (ab110132)
ab110132 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"