The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue.
Troponin I, fast twitch skeletal muscle isoform antibody
Troponin I, skeletal, fast antibody
Troponin I, slow skeletal muscle antibody
Troponin I, slow twitch isoform antibody
Anti-Troponin I fast + slow skeletal muscle antibody [12F10] images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Troponin I fast + slow skeletal muscle antibody [12F10] (ab110132)
ab110132 at 10 µg/ml staining Troponin I fast + slow skeletal muscle in Formalin-Fixed, Paraffin-Embedded Human skeletal muscle tissue by Immunohistochemistry. Detection utilised biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.