Anti-Troponin I fast + slow skeletal muscle antibody [12F10] (ab110132)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Troponin I fast + slow skeletal muscle antibody [12F10]
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DescriptionMouse monoclonal [12F10] to Troponin I fast + slow skeletal muscle
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Host speciesMouse
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SpecificityCross-reactivity with Cardiac Troponin I is less than 0.05%.
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Mouse
Predicted to work with: Human
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Immunogen
Mouse Troponin I fast + slow skeletal muscle.
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Positive control
- Human skeletal muscle tissue.
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General notes
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99.9% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone number12F10
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IsotypeIgG2b
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab110132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 21 kDa. | |
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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RelevanceTroponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue.
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Cellular localizationCytoplasmic and Nuclear
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Database links
- Entrez Gene: 7135 Human
- Entrez Gene: 7136 Human
- Entrez Gene: 21952 Mouse
- Entrez Gene: 21953 Mouse
- Omim: 191042 Human
- Omim: 191043 Human
- SwissProt: P19237 Human
- SwissProt: P48788 Human
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Alternative names
- AMCD2B antibody
- DA2B antibody
- DKFZp451O223 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Troponin I fast + slow skeletal muscle antibody [12F10] (ab110132)](http://a.abcam.com/ps/products/110/ab110132/Images/Troponin-I-fast-slow-skeletal-muscle-Primary-antibodies-ab110132-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Troponin I fast + slow skeletal muscle antibody [12F10] (ab110132)ab110132 at 10 µg/ml staining Troponin I fast + slow skeletal muscle in Formalin-Fixed, Paraffin-Embedded Human skeletal muscle tissue by Immunohistochemistry. Detection utilised biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
References
ab110132 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"