Anti-Troponin T1/TNT antibody (ab155028)
Key features and details
- Rabbit polyclonal to Troponin T1/TNT
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-Troponin T1/TNT antibody
See all Troponin T1/TNT primary antibodies -
Description
Rabbit polyclonal to Troponin T1/TNT -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant fragment corresponding to Human Troponin T1/TNT aa 1-278.
Database link: P13805 -
Positive control
- H1299 whole cell lysate. Human colon carcinoma and pig skeletal muscle tissue.
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General notes
Keep as concentrated solution.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 33 kDa.
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IHC-P | (1) |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 33 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. -
Involvement in disease
Nemaline myopathy 5 -
Sequence similarities
Belongs to the troponin T family. - Information by UniProt
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Database links
- Entrez Gene: 7138 Human
- Entrez Gene: 396579 Pig
- Omim: 191041 Human
- SwissProt: P13805 Human
- SwissProt: Q75ZZ6 Pig
- Unigene: 631558 Human
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Alternative names
- ANM antibody
- MGC104241 antibody
- NEM5 antibody
see all
Images
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Anti-Troponin T1/TNT antibody (ab155028) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 33 kDa
10% SDS PAGE -
Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue, labeling Troponin T1/TNT using ab155028 at 1/500 dilution.
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Immunohistochemical analysis of paraffin embedded Pig skeletal muscle tissue, labeling Troponin T1/TNT using ab155028 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab155028 has been referenced in 1 publication.
- Lardenois A et al. Quantitative proteome profiling of dystrophic dog skeletal muscle reveals a stabilized muscular architecture and protection against oxidative stress after systemic delivery of MuStem cells. Proteomics 16:2028-42 (2016). PubMed: 27246553